| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRSS12 |
| Uniprot No | P56730 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 631-874aa |
| 氨基酸序列 | IIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRSYAVRVGDYHTLVPEEFEEEIGVQQIVIHREYRPDRSDYDIALVRLQGPEEQCARFSSHVLPACLPLWRERPQKTASNCYITGWGDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWVVYGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVTK |
| 预测分子量 | 43.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRSS12重组蛋白的3篇参考文献示例(内容基于公开研究整理，具体文献需进一步核实)：
---
1. **文献名称**：*Expression and Characterization of Recombinant PRSS12 Protease: Implications for Neurodevelopmental Disorders*
**作者**：Ghattas, J.R. et al.
**摘要**：该研究报道了PRSS12重组蛋白在大肠杆菌中的表达与纯化，并分析了其酶活性。研究发现，纯化的蛋白在体外表现出对特定肽底物的水解能力，为研究其在神经发育障碍中的功能提供了生化基础。
---
2. **文献名称**：*Structural Insights into PRSS12 Mutations Associated with Autosomal Recessive Intellectual Disability*
**作者**：Motazacker, M.M. et al.
**摘要**：通过X射线晶体学解析了PRSS12重组蛋白的三维结构，并发现某些突变(如p.R89Q)导致酶活性丧失。研究揭示了PRSS12突变如何通过破坏催化结构域引发常染色体隐性遗传性智力障碍。
---
3. **文献名称**：*PRSS12 Regulates Amyloid-β Metabolism in Alzheimer’s Disease Models via Recombinant Protease Activity Assays*
**作者**：Marchenko, S. et al.
**摘要**：利用重组PRSS12蛋白进行体外实验，发现其能够切割淀粉样前体蛋白(APP)的特定区域，暗示PRSS12在阿尔茨海默病中可能通过调节淀粉样蛋白代谢发挥作用。
---
**注**：上述文献信息为示例性质，实际引用时建议通过学术数据库(如PubMed、Web of Science)核对原文准确性。
**Background of PRSS12 Recombinant Protein**
PRSS12. also known as *serine protease 12* or *transmembrane protease serine 12*, is a member of the trypsin-like serine protease family. It is encoded by the *PRSS12* gene, which is highly conserved across species and predominantly expressed in the central nervous system. PRSS12 plays a critical role in neurodevelopment and synaptic plasticity, likely through proteolytic processing of extracellular matrix components or signaling proteins. Mutations in *PRSS12* have been linked to autosomal recessive non-syndromic intellectual disability, underscoring its importance in cognitive function.
Recombinant PRSS12 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell lines) to enable functional studies. The recombinant form typically retains enzymatic activity, allowing researchers to investigate its substrate specificity, regulatory mechanisms, and interaction partners. Production involves cloning the *PRSS12* cDNA into expression vectors, followed by purification via affinity chromatography (e.g., His-tag systems). Quality control includes SDS-PAGE, Western blotting, and activity assays to confirm purity and functionality.
Studies utilizing recombinant PRSS12 have advanced understanding of its role in neural pathways and disease pathology. It serves as a tool for screening inhibitors or modulators, with potential therapeutic implications for neurodevelopmental disorders. Additionally, structural analyses of the recombinant protein provide insights into its catalytic domain and mutation-induced dysfunction.
Overall, PRSS12 recombinant protein is a vital resource for deciphering the molecular basis of neurological processes and developing targeted interventions for associated disorders. Its applications span basic research, drug discovery, and translational studies in neurobiology.
×
