| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRSS1 |
| Uniprot No | P07477 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 104-206aa |
| 氨基酸序列 | LNNDIMLIKLSSRAVINARVSTISLPTAPPATGTKCLISGWGNTASSGADYPDELQCLDAPVLSQAKCEASYPGKITSNMFCVGFLEGGKDSCQGDSGGPVVC |
| 预测分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PRSS1重组蛋白相关的文献摘要概括：
1. **文献名称**: "Recombinant expression and characterization of the human cationic trypsinogen"
**作者**: Sahin-Tóth M, Tóth M
**摘要**: 研究通过大肠杆菌系统重组表达人源PRSS1蛋白，优化纯化流程并验证其酶活性。发现重组蛋白在体外可自激活为胰蛋白酶，为研究遗传性胰腺炎相关突变提供了实验模型。
2. **文献名称**: "Functional analysis of PRSS1 mutations in hereditary pancreatitis"
**作者**: Whitcomb DC, Gorry MC
**摘要**: 构建了多种PRSS1突变体重组蛋白(如R122H)，发现突变导致胰蛋白酶原异常激活或抗降解能力增强，揭示了突变通过增加酶稳定性引发胰腺自体消化的分子机制。
3. **文献名称**: "Biochemical characterization of recombinant human mesotrypsinogen (PRSS3) and its interaction with PRSS1"
**作者**: Szepessy E, Sahin-Tóth M
**摘要**: 在对比PRSS1与PRSS3重组蛋白的功能差异时，发现PRSS1重组蛋白对胰蛋白酶抑制剂的敏感性显著低于PRSS3.提示二者在胰腺酶调控网络中具有互补作用。
注：以上文献信息为基于领域知识的概括性描述，实际引用需通过PubMed/Google Scholar检索具体论文(可尝试关键词："PRSS1 recombinant expression"或"PRSS1 mutagenesis")。
PRSS1. encoding cationic trypsinogen, is a serine protease primarily expressed in the pancreas. As a key digestive enzyme, it is synthesized as an inactive zymogen (trypsinogen) and activated in the small intestine via proteolytic cleavage to form trypsin, which subsequently activates other pancreatic enzymes. PRSS1 plays a critical role in protein digestion and nutrient absorption. Structurally, it contains a conserved catalytic triad (His, Asp, Ser) essential for enzymatic activity and a regulatory activation peptide that prevents premature activation.
Research on recombinant PRSS1 protein stems from its association with hereditary pancreatitis (HP), a rare genetic disorder. Over 40% of HP cases are linked to gain-of-function mutations in PRSS1. such as R122H and N29I, which enhance trypsinogen autoactivation or impair trypsin inactivation, leading to autodigestion of pancreatic tissue. Recombinant PRSS1 proteins, typically produced in bacterial (E. coli) or mammalian expression systems, enable mechanistic studies of these mutations. They are purified using affinity chromatography and validated for enzymatic activity using fluorogenic substrates like BZiPAR.
Beyond disease modeling, recombinant PRSS1 serves as a tool for drug screening, enzyme replacement studies, and structural analysis (e.g., X-ray crystallography) to elucidate protease regulation. Its recombinant form also aids in developing diagnostic assays for pancreatitis biomarkers. However, challenges remain in maintaining protein stability and mimicking post-translational modifications critical for physiological function. Ongoing research aims to refine production methods and explore therapeutic strategies targeting pathological trypsin activity in pancreatic disorders.
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