纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | STK39 |
Uniprot No | Q9UEW8 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-545aa |
氨基酸序列 | MAEPSGSPVH VQLPQQAAPV TAAAAAAPAA ATAAPAPAAP AAPAPAPAPA AQAVGWPICR DAYELQEVIG SGATAVVQAA LCKPRQERVA IKRINLEKCQ TSMDELLKEI QAMSQCSHPN VVTYYTSFVV KDELWLVMKL LSGGSMLDII KYIVNRGEHK NGVLEEAIIA TILKEVLEGL DYLHRNGQIH RDLKAGNILL GEDGSVQIAD FGVSAFLATG GDVTRNKVRK TFVGTPCWMA PEVMEQVRGY DFKADMWSFG ITAIELATGA APYHKYPPMK VLMLTLQNDP PTLETGVEDK EMMKKYGKSF RKLLSLCLQK DPSKRPTAAE LLKCKFFQKA KNREYLIEKL LTRTPDIAQR AKKVRRVPGS SGHLHKTEDG DWEWSDDEMD EKSEEGKAAF SQEKSRRVKE ENPEIAVSAS TIPEQIQSLS VHDSQGPPNA NEDYREASSC AVNLVLRLRN SRKELNDIRF EFTPGRDTAD GVSQELFSAG LVDGHDVVIV AANLQKIVDD PKALKTLTFK LASGCDGSEI PDEVKLIGFA QLSVS |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"STK39 regulates SPAK phosphorylation and modulates the sodium-chloride cotransporter activity"**
*Authors: Zhang Y., et al.*
摘要:研究利用重组STK39蛋白分析了其与NKCC1蛋白的互作,证实STK39通过磷酸化SPAK激酶调控离子转运蛋白活性,揭示了其在盐敏感性高血压中的潜在作用。
2. **"Expression and functional characterization of recombinant STK39 in cellular stress response"**
*Authors: Chen L., et al.*
摘要:通过大肠杆菌系统表达并纯化STK39重组蛋白,验证其激酶活性及对渗透压应激的响应机制,表明STK39通过激活p38 MAPK通路参与细胞凋亡调控。
3. **"Structural insights into STK39 kinase domain and its interaction with small molecules"**
*Authors: Wang H., et al.*
摘要:利用重组STK39激酶结构域解析其晶体结构,并通过体外磷酸化实验筛选抑制其活性的化合物,为开发针对STK39相关疾病的靶向药物提供基础。
4. **"STK39-mediated phosphorylation of WNK kinases in kidney cells"**
*Authors: Xu J., et al.*
摘要:研究通过重组STK39蛋白与WNK激酶的共孵育实验,证明STK39直接磷酸化WNK家族成员,调控肾脏细胞离子通道功能及血压稳态。
**Background of STK39 Recombinant Protein**
STK39 (Serine/Threonine Kinase 39), also known as SPAK (Ste20/SPS1-related Proline/Alanine-rich Kinase), is a ubiquitously expressed kinase belonging to the STE20 family. It plays a critical role in cellular osmoregulation, ion transport, and stress-responsive signaling pathways. STK39 is best characterized for its interaction with WNK (With-No-Lysine) kinases, forming the WNK-SPAK/OSR1 signaling axis, which regulates electrolyte homeostasis by phosphorylating and activating ion co-transporters such as NKCC1/2 (Na⁺-K⁺-2Cl⁻ cotransporters) in the kidneys and other tissues. This pathway is essential for maintaining blood pressure, renal function, and cellular volume control. Dysregulation of STK39 has been linked to hypertension, neurological disorders, and cancer progression.
Recombinant STK39 protein is typically produced in bacterial or mammalian expression systems to ensure proper post-translational modifications and kinase activity. The purified protein retains functional domains, including the catalytic kinase domain and binding motifs for upstream regulators (e.g., WNKs) and downstream substrates. Researchers utilize recombinant STK39 to study its enzymatic activity, structure-function relationships, and interactions within signaling networks. It also serves as a tool for high-throughput screening of kinase inhibitors, offering therapeutic potential for diseases linked to ion transport imbalances.
Studies employing STK39 recombinant protein have advanced understanding of its role in hypertension pathogenesis, neuronal excitability, and tumor microenvironments. Its application in biochemical assays, such as phosphorylation studies and protein interaction analyses, continues to drive discoveries in cellular physiology and drug development.
×