| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNN2 |
| Uniprot No | Q99439 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSTQFN KGPSYGLSAE VKNRLLSKYD PQKEAELRTW IEGLTGLSIG PDFQKGLKDG TILCTLMNKL QPGSVPKINR SMQNWHQLEN LSNFIKAMVS YGMNPVDLFE ANDLFESGNM TQVQVSLLAL AGKA |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNN2(Calponin 2)重组蛋白的假设参考文献示例，内容基于合理推测，实际文献需通过学术数据库验证：
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1. **文献名称**: *"Recombinant Calponin 2 inhibits cell migration via modulation of actin cytoskeleton dynamics"*
**作者**: Smith J, et al. (2015)
**摘要**: 本研究利用大肠杆菌表达系统成功制备重组CNN2蛋白，并证明其通过结合肌动蛋白微丝调控细胞骨架重塑。体外实验表明，外源性重组CNN2可显著抑制HeLa细胞的迁移能力，提示其在细胞运动相关病理过程中的潜在作用。
2. **文献名称**: *"Optimization of expression and purification of human CNN2 in E. coli for structural studies"*
**作者**: Zhang L, et al. (2018)
**摘要**: 报道了一种改良的重组CNN2蛋白表达纯化方案，通过密码子优化和温度调控提高蛋白可溶性。纯化的CNN2保留了天然构象，为后续X射线晶体学分析及功能研究提供了高质量样本。
3. **文献名称**: *"Phosphorylation-dependent interaction of recombinant calponin 2 with smooth muscle myosin"*
**作者**: Lee S, et al. (2020)
**摘要**: 通过体外重构实验，证实重组CNN2与平滑肌肌球蛋白的结合受PKC介导的磷酸化调控。该互作可能通过影响收缩蛋白组装参与血管张力调节，为高血压机制研究提供了新视角。
4. **文献名称**: *"Recombinant CNN2 suppresses metastatic potential in breast cancer cells through RhoA/ROCK pathway inhibition"*
**作者**: Patel R, Johnson M (2022)
**摘要**: 研究显示，重组CNN2蛋白处理可下调乳腺癌细胞中RhoA活性，减少伪足形成和基质侵袭，提示其作为转移抑制因子的治疗潜力，并建立了基于荧光标记CNN2的动态细胞成像方法。
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**注**: 以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台以关键词"Calponin 2 recombinant"或"CNN2 expression"检索。建议结合研究具体方向(如疾病模型、信号通路等)进一步筛选文献。
CNN2 (Calponin-2) is a cytoskeletal protein belonging to the calponin family, known for its role in regulating actin filament dynamics and smooth muscle contraction. It is expressed in various tissues, including smooth muscle cells, endothelial cells, and certain non-muscle cells, where it modulates cell motility, adhesion, and mechanical stability. The protein contains conserved calponin homology (CH) domains that mediate interactions with actin and other signaling molecules, influencing cellular contractility and mechanotransduction pathways.
Recombinant CNN2 protein is produced using genetic engineering techniques, typically through expression in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells). This allows for large-scale purification of the protein with high homogeneity, enabling detailed structural and functional studies. Recombinant CNN2 retains its biochemical properties, such as actin-binding capacity and phosphorylation-dependent regulatory functions, making it a valuable tool for *in vitro* assays.
Research on CNN2 has gained attention due to its involvement in pathological conditions. It is implicated in cardiovascular diseases, such as hypertension and atherosclerosis, by affecting vascular smooth muscle cell behavior. Additionally, CNN2 dysregulation is linked to cancer progression, particularly in tumor cell invasion and metastasis, where it modulates cytoskeletal remodeling and extracellular matrix interactions. Studies using recombinant CNN2 have shed light on its role in TGF-β signaling, inflammation, and fibrosis, highlighting its potential as a therapeutic target.
The development of CNN2 recombinant protein has also facilitated antibody production, drug screening, and structural analysis (e.g., X-ray crystallography or cryo-EM), advancing our understanding of its molecular mechanisms. Ongoing research aims to explore its interplay with other cytoskeletal regulators and its tissue-specific functions in health and disease.
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