| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AQP3 |
| Uniprot No | Q92482 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MGRQKELVSRCGEMLHIRYRLLRQALAECLGTLILVMFGCGSVAQVVLSRGTHGGFLTINLAFGFAVTLGILIAGQVSGAHLNPAVTFAMCFLAREPWIKLPIYTLAQTLGAFLGAGIVFGLYYDAIWHFADNQLFVSGPNGTAGIFATYPSGHLDMINGFFDQFIGTASLIVCVLAIVDPYNNPVPRGLEAFTVGLVVLVIGTSMGFNSGYAVNPARDFGPRLFTALAGWGSAVFTTGQHWWWVPIVSPLLGSIAGVFVYQLMIGCHLEQPPPSNEEENVKLAHVKHKEQI |
| 预测分子量 | 31,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AQP3重组蛋白的3篇示例参考文献(注：内容为模拟，非真实文献)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant Human AQP3 in a Mammalian Cell System*
**作者**：Smith J, et al.
**摘要**：研究通过哺乳动物细胞系统表达重组人AQP3蛋白，证实其在细胞膜上介导水和甘油转运的功能，并发现其在渗透调节中的作用。
2. **文献名称**：*Structural Characterization of AQP3 Recombinant Protein Using Cryo-Electron Microscopy*
**作者**：Wang L, et al.
**摘要**：利用冷冻电镜技术解析AQP3重组蛋白的三维结构，揭示了其通道选择性及与脂质双分子层的相互作用机制。
3. **文献名称**：*AQP3 Recombinant Protein Promotes Skin Hydration in a Diabetic Mouse Model*
**作者**：Chen Y, et al.
**摘要**：在小鼠模型中验证重组AQP3蛋白的皮肤渗透性功能，证明其通过增强角质细胞水分运输改善糖尿病相关的皮肤干燥。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“AQP3 recombinant protein”获取。
Aquaporin 3 (AQP3), a member of the aquaporin family, is a transmembrane channel protein that facilitates the transport of water and small solutes like glycerol and urea. Discovered in the 1990s, AQP3 is distinct from other aquaporins due to its dual permeability to water and nonpolar molecules, a feature critical for its role in cellular hydration, osmoregulation, and energy metabolism. It is widely expressed in epithelial and endothelial tissues, including the skin, kidneys, gastrointestinal tract, and respiratory system, where it maintains fluid homeostasis and supports barrier function.
Recombinant AQP3 protein is engineered using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification via affinity chromatography. This engineered protein retains the functional properties of native AQP3. enabling researchers to study its structure, transport mechanisms, and interactions in controlled environments. Its applications span basic research—investigating osmotic regulation, cell proliferation, and wound healing—and drug development, particularly for conditions linked to AQP3 dysfunction, such as skin disorders (e.g., xerosis, psoriasis), renal diseases, or metabolic syndromes.
Recent studies highlight AQP3's involvement in cancer progression, as its overexpression in certain tumors correlates with enhanced cell migration and chemoresistance. Recombinant AQP3 also serves as a tool for screening therapeutic agents targeting channel activity. Despite advances, challenges remain in stabilizing the protein for *in vivo* applications and understanding its regulatory networks. Ongoing research aims to harness recombinant AQP3 for innovative therapies, including topical formulations for dermatological repair or systemic modulators for metabolic diseases.
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