| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AQP2 |
| Uniprot No | P41181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGL GIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAG AALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTD ERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDD HWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEERE VRRRQSVELHSPQSLPRGTKA |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AQP2重组蛋白的参考文献示例(注：以下内容为示例，实际文献需通过学术数据库查询)：
1. **标题**: "Expression and Functional Characterization of Recombinant Human Aquaporin-2 in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 研究利用HEK293细胞表达重组人AQP2蛋白，证实其水通道活性受血管加压素调控，并通过细胞膜转运实验证明磷酸化对其功能的关键作用。
2. **标题**: "Purification and Structural Analysis of AQP2 Using a Bacterial Expression System"
**作者**: Tanaka K, et al.
**摘要**: 通过大肠杆菌系统表达带His标签的AQP2重组蛋白，优化纯化条件并利用冷冻电镜解析其三维结构，揭示其水选择性过滤的分子机制。
3. **标题**: "Role of AQP2 Mutants in Nephrogenic Diabetes Insipidus: Insights from Recombinant Protein Studies"
**作者**: Chen L, et al.
**摘要**: 构建多个AQP2突变体重组蛋白，发现部分突变导致蛋白错误折叠和膜定位缺陷，解释了遗传性尿崩症的分子病理机制。
4. **标题**: "Post-translational Modification of Recombinant AQP2: Impact on Water Permeability"
**作者**: Müller D, et al.
**摘要**: 研究重组AQP2的磷酸化及泛素化修饰，发现特定位点修饰调控其细胞内运输及稳定性，影响肾小管细胞的水通透性。
建议通过PubMed或Google Scholar检索关键词“AQP2 recombinant protein”获取最新真实文献。
Aquaporin-2 (AQP2) is a member of the aquaporin family of water channel proteins, primarily expressed in the principal cells of the renal collecting ducts. It plays a critical role in regulating water reabsorption and maintaining body water homeostasis. AQP2 facilitates the translocation of water across cell membranes in response to osmotic gradients, a process tightly controlled by the antidiuretic hormone arginine vasopressin (AVP). Upon AVP binding to the V2 receptor on renal cells, intracellular cAMP signaling triggers the trafficking of AQP2-containing vesicles to the apical membrane, increasing membrane permeability to water. This mechanism allows the kidneys to concentrate urine and adjust water retention according to physiological needs.
Recombinant AQP2 protein is engineered through molecular cloning techniques, typically expressed in heterologous systems such as Escherichia coli, yeast, or mammalian cell lines (e.g., HEK293). These systems enable large-scale production of the protein for structural and functional studies. Recombinant AQP2 retains key structural features, including six transmembrane domains, two conserved asparagine-proline-alanine (NPA) motifs essential for water selectivity, and phosphorylation sites critical for regulatory trafficking.
Research on recombinant AQP2 has advanced understanding of its role in pathophysiology. Mutations in the AQP2 gene are linked to nephrogenic diabetes insipidus (NDI), a disorder characterized by impaired water reabsorption and severe dehydration. Recombinant variants mimicking these mutations help elucidate molecular defects, such as misfolding or misrouting, and screen potential therapeutic agents (e.g., pharmacological chaperones). Additionally, AQP2 is implicated in conditions like heart failure and syndrome of inappropriate antidiuretic hormone secretion (SIADH), where dysregulation contributes to water imbalance.
Overall, recombinant AQP2 serves as a vital tool for dissecting water channel biology, modeling diseases, and developing targeted therapies to restore aquaporin function in related disorders.
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