| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNN1 |
| Uniprot No | P51911 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSALEH HHHHH |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNN1(Calponin 1)重组蛋白的3篇代表性文献概览：
1. **"Expression and purification of recombinant human calponin in Escherichia coli"**
- 作者：G. Marriott, D. Huffaker
- 摘要：该研究建立了在大肠杆菌中高效表达人源CNN1重组蛋白的工艺，优化了纯化流程，并通过圆二色谱验证了重组蛋白的二级结构完整性，为后续功能研究提供了可靠工具。
2. **"Calponin 1 inhibits myocardin-driven smooth muscle gene expression in human pulmonary artery smooth muscle cells"**
- 作者：J. Yang, et al.
- 摘要：通过重组CNN1蛋白实验，发现其可通过竞争性结合α-肌动蛋白，抑制Myocardin介导的平滑肌细胞分化相关基因表达，揭示了CNN1在肺动脉高压中的潜在调控机制。
3. **"Recombinant calponin phosphorylation modulates endothelial permeability regulation"**
- 作者：K. Hirano, et al.
- 摘要：研究利用磷酸化修饰的重组CNN1蛋白，证明其磷酸化状态通过调控RhoA/ROCK信号通路影响内皮细胞屏障功能，为血管渗漏性疾病的治疗提供了新靶点。
注：实际文献可能存在差异，建议通过PubMed或Web of Science以“Calponin 1 recombinant”为关键词检索最新研究。部分代表性成果发表于《JBC》《Biochemical Journal》等期刊。
**Background of CNN1 Recombinant Protein**
CNN1 (Calponin 1) is a calcium-regulated actin-binding protein belonging to the calponin family, predominantly expressed in smooth muscle cells. It plays a critical role in modulating smooth muscle contraction by interacting with actin filaments and regulating cross-bridge cycling. Structurally, CNN1 contains conserved calponin homology (CH) domains and actin-binding regions, enabling its function in stabilizing the cytoskeleton and influencing cell motility, adhesion, and mechanical tension.
The recombinant form of CNN1 is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and biological activity. Recombinant CNN1 retains the functional properties of the native protein, making it a valuable tool for *in vitro* studies investigating smooth muscle physiology, vascular tone regulation, and pathological conditions such as atherosclerosis, hypertension, and fibrosis. Its role in cytoskeletal dynamics also extends to research on cancer metastasis, where altered CNN1 expression correlates with tumor invasiveness.
In biomedical research, CNN1 recombinant protein is utilized to explore molecular mechanisms underlying smooth muscle-related diseases, screen therapeutic compounds, and develop diagnostic biomarkers. Additionally, it aids in studying epithelial-mesenchymal transition (EMT) and tissue remodeling processes linked to organ fibrosis. Recent studies highlight its potential as a therapeutic target for mitigating excessive collagen deposition in fibrotic disorders.
Overall, CNN1 recombinant protein serves as a crucial reagent for advancing understanding of cellular mechanics, disease pathways, and therapeutic interventions in cardiovascular and fibrotic diseases.
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