| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPO5 |
| Uniprot No | Q9HAV4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1204aa |
| 氨基酸序列 | MAMDQVNALCEQLVKAVTVMMDPNSTQRYRLEALKFCEEFKEKCPICVPCGLRLAEKTQVAIVRHFGLQILEHVVKFRWNGMSRLEKVYLKNSVMELIANGTLNILEEENHIKDALSRIVVEMIKREWPQHWPDMLIELDTLSKQGETQTELVMFILLRLAEDVVTFQTLPPQRRRDIQQTLTQNMERIFSFLLNTLQENVNKYQQVKTDTSQESKAQANCRVGVAALNTLAGYIDWVSMSHITAENCKLLEILCLLLNEQELQLGAAECLLIAVSRKGKLEDRKPLMVLFGDVAMHYILSAAQTADGGGLVEKHYVFLKRLCQVLCALGNQLCALLGADSDVETPSNFGKYLESFLAFTTHPSQFLRSSTQMTWGALFRHEILSRDPLLLAIIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFDSDEDFNAFFNSSRAQQGEVMRLACRLDPKTSFQMAGEWLKYQLSTFLDAGSVNSCSAVGTGEGSLCSVFSPSFVQWEAMTLFLESVITQMFRTLNREEIPVNDGIELLQMVLNFDTKDPLILSCVLTNVSALFPFVTYRPEFLPQVFSKLFSSVTFETVEESKAPRTRAVRNVRRHACSSIIKMCRDYPQLVLPNFDMLYNHVKQLLSNELLLTQMEKCALMEALVLISNQFKNYERQKVFLEELMAPVASIWLSQDMHRVLSDVDAFIAYVGTDQKSCDPGLEDPCGLNRARMSFCVYSILGVVKRTCWPTDLEEAKAGGFVVGYTSSGNPIFRNPCTEQILKLLDNLLALIRTHNTLYAPEMLAKMAEPFTKALDMLDAEKSAILGLPQPLLELNDSPVFKTVLERMQRFFSTLYENCFHILGKAGPSMQQDFYTVEDLATQLLSSAFVNLNNIPDYRLRPMLRVFVKPLVLFCPPEHYEALVSPILGPLFTYLHMRLSQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRMLTREVMDLITVCCVSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDLGKCLMKHEDVCTALLITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTLLADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLLNPSLQKVADKRRKDQFKRLIAGCIGKPLGEQFRKEVHIKNLPSLFKKTKPMLETEVLDNDGGGLATIFEP |
| 预测分子量 | 136,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇与XPO5重组蛋白相关的虚构参考文献示例(仅供格式参考，非真实文献)：
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1. **文献名称**: *Structural insights into the RanGTP-dependent nuclear export of pre-miRNA by Exportin-5*
**作者**: Zhang Y. et al.
**摘要**: 通过X射线晶体学解析了XPO5与RanGTP及pre-miRNA结合的复合物结构，揭示了XPO5特异性识别pre-miRNA茎环结构的分子机制，为设计调控miRNA运输的小分子提供依据。
2. **文献名称**: *Optimization of recombinant XPO5 expression in E. coli for functional studies*
**作者**: Smith J. et al.
**摘要**: 报道了一种在大肠杆菌中高效表达并纯化功能性重组XPO5蛋白的方案，通过优化诱导条件和亲和层析步骤，获得高纯度蛋白用于体外转运实验。
3. **文献名称**: *XPO5 dysfunction promotes oncogenic miRNA accumulation in colorectal cancer*
**作者**: Lee H. et al.
**摘要**: 利用重组XPO5蛋白进行功能挽救实验，证明XPO5的突变导致pre-miRNA核滞留，促进致癌性miR-21的异常表达，与结直肠癌进展相关。
4. **文献名称**: *Development of a FRET-based assay for XPO5 inhibitor screening using recombinant protein*
**作者**: Johnson R. et al.
**摘要**: 基于重组XPO5蛋白构建了一种高通量荧光共振能量转移(FRET)检测体系，用于筛选靶向XPO5-RanGTP结合界面的抑制剂，发现多个先导化合物。
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注：以上文献为示例性内容，实际研究中请通过数据库(如PubMed)检索真实文献。
**Background of XPO5 Recombinant Protein**
XPO5 (Exportin-5) is a critical member of the karyopherin-β family of nuclear transport proteins, primarily responsible for mediating the export of small RNAs, including precursor microRNAs (pre-miRNAs) and certain small nuclear RNAs, from the nucleus to the cytoplasm. This process is essential for post-transcriptional gene regulation, as mature microRNAs (miRNAs) bind to target mRNAs to inhibit translation or promote degradation.
The XPO5 protein functions by recognizing and binding to its cargoes through a RanGTP-dependent mechanism. Structurally, XPO5 contains characteristic HEAT repeats that facilitate interactions with cargo and RanGTP. Dysregulation of XPO5 has been implicated in various diseases, including cancer, where aberrant miRNA export can alter tumor suppressor or oncogenic pathways.
Recombinant XPO5 protein is engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce a purified, functional form of the protein for research and therapeutic applications. It retains the ability to bind pre-miRNAs and RanGTP, enabling studies on nuclear-cytoplasmic transport mechanisms, miRNA biogenesis, and disease pathology. Researchers utilize XPO5 recombinant protein in *in vitro* assays, structural analyses, and drug discovery, particularly for developing inhibitors targeting dysregulated export pathways in cancers.
Its role in maintaining RNA homeostasis and disease associations makes XPO5 a compelling target for biomedical research, with recombinant variants serving as vital tools for understanding cellular transport dynamics and exploring therapeutic interventions.
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