| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNDP2 |
| Uniprot No | Q96KP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAALTTL FKYIDENQDR YIKKLAKWVA IQSVSAWPEK RGEIRRMMEV AAADVKQLGG SVELVDIGKQ KLPDGSEIPL PPILLGRLGS DPQKKTVCIY GHLDVQPAAL EDGWDSEPFT LVERDGKLYG RGSTDDKGPV AGWINALEAY QKTGQEIPVN VRFCLEGMEE SGSEGLDELI FARKDTFFKD VDYVCISDNY WLGKKKPCIT YGLRGICYFF IEVECSNKDL HSGVYGGSVH EAMTDLILLM GSLVDKRGNI LIPGINEAVA AVTEEEHKLY DDIDFDIEEF AKDVGAQILL HSHKKDILMH RWRYPSLSLH GIEGAFSGSG AKTVIPRKVV GKFSIRLVPN MTPEVVGEQV TSYLTKKFAE LRSPNEFKVY MGHGGKPWVS DFSHPHYLAG RRAMKTVFGV EPDLTREGGS IPVTLTFQEA TGKNVMLLPV GSADDGAHSQ NEKLNRYNYI EGTKMLAAYL YEVSQLKD |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNDP2重组蛋白的示例性参考文献(注：文献为模拟示例，仅供参考格式)：
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1. **文献名称**: *Recombinant Expression and Enzymatic Characterization of Human CNDP2 in E. coli*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中成功表达并纯化重组人源CNDP2蛋白，通过体外酶活实验证实其水解肌肽(carnosine)的活性，为后续功能研究提供工具。
2. **文献名称**: *Structural Insights into CNDP2 via X-ray Crystallography Using Recombinant Protein*
**作者**: Johnson R, et al.
**摘要**: 利用昆虫细胞系统表达重组CNDP2蛋白，解析其晶体结构，揭示了底物结合口袋的构象变化，为设计靶向抑制剂奠定基础。
3. **文献名称**: *Role of Recombinant CNDP2 in Modulating Cancer Cell Metabolism*
**作者**: Lee S, et al.
**摘要**: 通过体外实验证明重组CNDP2蛋白通过调控组氨酸代谢影响肿瘤细胞增殖，提示其在癌症治疗中的潜在靶点价值。
4. **文献名称**: *CNDP2 Recombinant Protein Attenuates Neurodegeneration in Alzheimer's Disease Models*
**作者**: Garcia M, et al.
**摘要**: 研究发现重组CNDP2蛋白可降解β淀粉样蛋白聚集，改善阿尔茨海默病模型中的神经元损伤，为神经保护机制提供新视角。
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如需真实文献，建议通过PubMed或Web of Science以关键词**CNDP2 recombinant**检索最新研究。
CNDP2 (Carnosine Dipeptidase 2), also known as CN2 or peptidase M20 domain-containing protein 2. is a zinc-dependent metallopeptidase belonging to the M20 family. It plays a critical role in hydrolyzing dipeptides, particularly carnosine (β-alanyl-L-histidine), into β-alanine and histidine, influencing cellular processes like antioxidant defense, pH regulation, and energy metabolism. CNDP2 is expressed in various tissues, including the liver, kidney, and brain, and has been linked to metabolic disorders, neurodegenerative diseases, and cancer progression. Its enzymatic activity and regulatory functions make it a target for studying conditions such as diabetes, aging, and tumorigenesis.
Recombinant CNDP2 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Purification methods like affinity chromatography (e.g., His-tag) yield high-purity protein for functional studies. Researchers utilize recombinant CNDP2 to investigate substrate specificity, enzyme kinetics, and interactions with inhibitors or therapeutic agents. It also aids in elucidating its role in disease pathways, such as modulating oxidative stress in diabetic complications or influencing cancer cell proliferation via carnosine metabolism. Additionally, recombinant CNDP2 serves as a tool for developing diagnostic assays or targeted therapies, highlighting its biomedical relevance. Recent studies further explore its potential as a biomarker or drug target, underscoring the importance of this protein in both basic and applied research.
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