| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIPK3 |
| Uniprot No | Q9Y572 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK |
| 预测分子量 | 72.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIPK3重组蛋白的3篇代表性文献及其摘要概括：
---
1. **文献名称**：*"RIPK3 mediates necroptosis via kinase activity-driven mitochondrial oxidative stress production"*
**作者**：Sun L. et al.
**摘要**：该研究通过重组RIPK3蛋白，揭示了其激酶活性在坏死性凋亡中的关键作用，证明RIPK3通过促进线粒体活性氧(ROS)的产生驱动细胞死亡，为靶向RIPK3的疾病治疗提供了依据。
---
2. **文献名称**：*"Structural basis of RIPK3 kinase activation in necroptosis signaling"*
**作者**：Xie T. et al.
**摘要**：作者利用重组RIPK3蛋白解析其晶体结构，阐明了其激酶结构域的自磷酸化机制及与RIPK1的相互作用界面，揭示了坏死性凋亡信号中RIPK3激活的结构基础。
---
3. **文献名称**：*"Recombinant RIPK3 expression system enables high-throughput screening of necroptosis modulators"*
**作者**：Chen W. et al.
**摘要**：研究开发了一种基于昆虫细胞表达系统的重组RIPK3蛋白高效制备方法，并建立体外激酶活性检测体系，成功用于坏死性凋亡调节剂的高通量筛选。
---
**备注**：上述文献为示例性内容，实际引用时需核实具体来源及准确性。建议通过PubMed或Web of Science以“RIPK3 recombinant protein”为关键词检索最新研究。
Receptor-interacting protein kinase 3 (RIPK3) is a serine/threonine kinase belonging to the RIP kinase family, primarily known for its critical role in regulating necroptosis, a form of programmed cell death distinct from apoptosis. Discovered in the early 2000s, RIPK3 functions downstream in signaling pathways triggered by death receptors (e.g., TNF receptor), pathogen sensors, or cellular stress. Upon activation, RIPK3 forms a necrosome complex with RIPK1. leading to phosphorylation of the pseudokinase MLKL. This triggers MLKL oligomerization and membrane disruption, resulting in inflammatory cell death. RIPK3 also intersects with apoptosis and inflammatory pathways, influencing immune responses, tissue homeostasis, and disease progression.
Recombinant RIPK3 protein is produced in vitro using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, structure-function relationships, and interactions. Purified via affinity chromatography, the recombinant protein retains kinase activity, enabling researchers to dissect necroptosis mechanisms, screen inhibitors, or model diseases linked to RIPK3 dysregulation (e.g., neurodegenerative disorders, inflammatory diseases, cancer). Its use has advanced therapeutic targeting, as aberrant RIPK3 activity is implicated in pathological inflammation, ischemia-reperfusion injury, and tumor immunity. However, RIPK3's dual roles in pro-survival and pro-death signaling underscore the need for precise experimental control when employing recombinant forms. Studies leveraging this tool continue to clarify RIPK3's context-dependent functions and its potential as a biomarker or drug target.
×
