| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNBP |
| Uniprot No | P62633-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSNECF KCGRSGHWAR ECPTGGGRGR GMRSRGRGFQ FVSSSLPDIC YRCGESGHLA KDCDLQEDAC YNCGRGGHIA KDCKEPKRER EQCCYNCGKP GHLARDCDHA DEQKCYSCGE FGHIQKDCTK VKCYRCGETG HVAINCSKTS EVNCYRCGES GHLARECTIE ATA |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNBP重组蛋白的3篇参考文献示例(注：文献为虚构，仅供格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant CNBP in Escherichia coli for Functional Studies*
**作者**：Smith J, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效生产人源CNBP重组蛋白的方法，优化了诱导条件以提高可溶性蛋白产量，并通过亲和层析纯化获得高纯度蛋白，为后续核酸结合活性分析提供了材料。
2. **文献名称**：*Structural Insights into CNBP’s Zinc Finger Domains by X-ray Crystallography*
**作者**：Johnson R, et al.
**摘要**：通过重组CNBP蛋白的结晶和X射线衍射分析，揭示了其锌指结构域的三维构象，并发现其与单链DNA/RNA结合的分子机制，提示其在基因表达调控中的潜在作用。
3. **文献名称**：*CNBP Knockdown and Recombinant Protein Rescue Experiments in Myotonic Dystrophy Models*
**作者**：Lee S, et al.
**摘要**：利用重组CNBP蛋白在细胞模型中恢复功能，证明其可逆转异常剪接事件，支持CNBP在强直性肌营养不良症中的病理相关性，为治疗策略提供依据。
(注：实际文献需通过PubMed、Web of Science等平台检索关键词如“CNBP recombinant”、“ZNBP1 protein purification”获取。)
Cellular Nucleic Acid Binding Protein (CNBP), also known as Zinc Finger Protein 9 (ZNF9), is a highly conserved, multifunctional protein found in vertebrates. It plays critical roles in transcriptional and translational regulation by binding to single-stranded nucleic acids, including DNA and RNA. CNBP contains seven zinc finger domains and an arginine-rich region, enabling interactions with G-rich sequences in promoters, untranslated regions (UTRs), or other structured nucleic acid motifs. These interactions influence processes such as cell proliferation, embryonic development, and neural crest cell differentiation. Notably, CNBP is essential for proper craniofacial and muscular development in vertebrates.
Recombinant CNBP proteins are engineered using expression systems like *E. coli* or mammalian cell cultures, allowing large-scale production for functional studies. The protein’s structure and nucleic acid-binding mechanisms have been investigated to clarify its role in diseases. For example, abnormal expansion of a repetitive DNA sequence in the *CNBP* gene is linked to myotonic dystrophy type 2 (DM2), a neuromuscular disorder characterized by RNA toxicity and mis-splicing of transcripts. Recombinant CNBP aids in studying its interaction with pathogenic RNA foci and potential therapeutic strategies.
Research on CNBP also extends to cancer and neurodegeneration, as dysregulation of nucleic acid metabolism or stress response pathways involving CNBP may contribute to pathology. Its conserved nature and diverse functions make CNBP a compelling target for understanding gene regulation and developing biomedical interventions.
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