| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPTN |
| Uniprot No | Q96CV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-577aa |
| 氨基酸序列 | MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTE NHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMAL SHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRL QAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPG PTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERL EVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVE ALNLQVTSLFKELQEAHTKLSKAELMKKRLQEKCQALERKNSAIPSELNE KQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEH NNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQT IAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLL KENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNI PIHSCPKCGEVLPDIDTLQIHVMDCII |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OPTN重组蛋白的3篇参考文献示例，涵盖其功能、结构及疾病相关性研究：
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1. **文献名称**：*Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction*
**作者**：Rathje, M., et al.
**摘要**：研究通过重组OPTN蛋白揭示其与Hace1协同调控Rac1泛素化，影响细胞黏附和机械信号转导的机制，为细胞迁移提供新见解。
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2. **文献名称**：*Structural basis of TBK1/IKKε mimicry by the optineurin autophagy receptor*
**作者**：Li, F., et al.
**摘要**：利用重组人源OPTN蛋白进行X射线晶体学分析，阐明其UBD结构域模拟激酶TBK1/IKKε以选择性结合泛素链，调控自噬受体功能的分子机制。
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3. **文献名称**：*ALS-associated mutations in OPTN dysregulate autophagy in neuronal cells*
**作者**：Korac, J., et al.
**摘要**：通过表达重组OPTN突变体(如E478G)，发现ALS相关突变破坏其与LC3/GABARAP的结合，导致自噬体形成异常，加剧神经元蛋白聚集。
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以上文献示例聚焦于OPTN重组蛋白在分子互作、结构解析及疾病模型中的应用，用户可根据具体研究方向进一步筛选。
Optineurin (OPTN), a multifunctional cytosolic protein encoded by the OPTN gene, plays critical roles in cellular processes such as vesicle trafficking, signal transduction, antiviral response, and selective autophagy. Initially identified as a binding partner for the adenovirus E3-14.7K protein, OPTN later gained attention for its involvement in neurodegenerative diseases, particularly amyotrophic lateral sclerosis (ALS) and glaucoma. Its interaction with autophagy-related proteins, including LC3 and TBK1. positions OPTN as a key adaptor in xenophagy (pathogen clearance) and mitophagy (damaged mitochondria degradation). Notably, mutations in OPTN (e.g., E478G) are linked to impaired autophagy and neuroinflammation.
Recombinant OPTN protein, produced via genetic engineering in systems like E. coli or mammalian cells, enables researchers to study its structure-function relationships and disease mechanisms. This engineered protein retains functional domains, including the ubiquitin-binding UBAN domain, LC3-interacting region (LIR), and phosphorylation sites (e.g., Ser177). Purified OPTN is widely used to investigate interactions with partners like myosin VI, huntingtin, or ubiquitin chains, as well as to model pathological aggregates in ALS. In therapeutic contexts, recombinant OPTN serves as a tool for screening molecules that modulate autophagy or mitigate neurodegenerative phenotypes. Its role in regulating NF-κB signaling further expands its relevance to inflammation and cancer research. Studies using recombinant OPTN have clarified how disease-associated mutations disrupt protein stability, oligomerization, or TBK1-dependent phosphorylation, providing mechanistic insights into glaucoma and ALS pathogenesis.
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