| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBP3 |
| Uniprot No | P10745 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1149-1246aa |
| 氨基酸序列 | GTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYLTIPTARSV GASDGSSWEGVGVTPHVVVPAEEALARAKEMLQHNQLRVKRSPGLQDH |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBP3(视黄醇结合蛋白3.也称为IRBP)重组蛋白研究的模拟参考文献示例。这些示例基于常见研究方向，具体文献需通过学术数据库进一步检索：
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1. **标题**：*"Expression and purification of recombinant human interphotoreceptor retinoid-binding protein (IRBP) in a mammalian cell system"*
**作者**：Gonzalez-Fernandez F, et al.
**摘要**：研究描述了在哺乳动物细胞中重组表达人源IRBP(RBP3)的方法，验证了其糖基化修饰及对视黄醇的结合活性，为研究视网膜疾病机制提供工具。
2. **标题**：*"Structural characterization of recombinant RBP3 and its role in retinal regeneration"*
**作者**：Li Y, et al.
**摘要**：通过X射线晶体学解析重组RBP3的三维结构，揭示其与视黄醇衍生物的相互作用位点，并证明其在体外促进光感受器细胞再生中的作用。
3. **标题**：*"Development of a prokaryotic expression system for high-yield production of bioactive RBP3"*
**作者**：Chen X, Wang L.
**摘要**：优化大肠杆菌表达体系，实现重组RBP3的高效可溶性表达，纯化蛋白在动物模型中展示出缓解维生素A缺乏相关眼病的潜力。
4. **标题**：*"Recombinant IRBP as a therapeutic agent in autoimmune uveitis: preclinical evaluation"*
**作者**：Singh AK, et al.
**摘要**：评估重组IRBP在自身免疫性葡萄膜炎模型中的免疫调节作用，证明其通过抑制T细胞反应减轻炎症损伤。
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**说明**：以上为模拟文献，实际研究中建议通过PubMed、Web of Science等平台检索关键词如 **"recombinant RBP3"**、**"IRBP expression"** 或 **"retinol-binding protein 3"** 获取真实文献。
Retinol-binding protein 3 (RBP3), also known as interphotoreceptor retinoid-binding protein (IRBP), is a glycoprotein critical to the visual cycle in vertebrates. Primarily synthesized and secreted by photoreceptor cells in the retina, it facilitates the transport of lipid-soluble retinoids (all-*trans* retinol and 11-*cis* retinal) between photoreceptors and retinal pigment epithelium (RPE). This process is essential for regenerating visual pigments after phototransduction, enabling light detection and vision maintenance. Structurally, RBP3 contains tandem repeats of conserved domains that bind retinoids and fatty acids, ensuring their solubility and protection in the aqueous interphotoreceptor matrix.
Recombinant RBP3 is produced using biotechnological platforms, such as *E. coli*, yeast, or mammalian cell systems, to express the protein with high purity and consistency. Its recombinant form retains native binding properties, making it invaluable for studying retinoid metabolism, photoreceptor-RPE interactions, and diseases like retinitis pigmentosa or age-related macular degeneration (AMD). Researchers also utilize recombinant RBP3 to develop therapeutic strategies, including drug delivery systems targeting retinal disorders or gene therapies to correct mutations affecting visual cycles.
Beyond basic research, recombinant RBP3 serves as a tool for diagnostic assays, aiding in biomarker discovery for early detection of retinal pathologies. Challenges in its application include maintaining post-translational modifications (e.g., glycosylation) critical for function, which often necessitates mammalian expression systems. Ongoing studies focus on optimizing production methods and exploring its neuroprotective roles in degenerative eye diseases. Overall, recombinant RBP3 bridges mechanistic insights into vision biology and translational innovations for ocular therapeutics.
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