关键信息
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基因名
G-CSFR/CD114
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简介
The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293, His-Flag) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-6*His, C-Flag labeled tag.
- 应用
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别名
colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor;
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种属
Human
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表达系统
HEK293
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标签
C-6*His;C-Flag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99062
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表达区间
E25-P621
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氨基酸序列
ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP
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蛋白长度
Partial
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分子量
Apparently 100 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
G-CSFR (Granulocyte Colony-Stimulating Factor Receptor) or CD114 is a crucial receptor involved in the regulation of hematopoiesis, particularly in the differentiation and proliferation of granulocyte precursors in the bone marrow. The binding of G-CSF (Granulocyte Colony-Stimulating Factor) to G-CSFR activates signaling pathways that promote the survival, proliferation, and maturation of neutrophil progenitors, essential for the body's immune response. Research on G-CSFR/CD114 recombinant proteins has gained prominence due to their potential therapeutic applications in treating various hematological disorders, such as neutropenia, particularly in patients undergoing chemotherapy or bone marrow disorders. Understanding the structure and function of G-CSFR/CD114 can provide insights into the mechanisms of neutrophil regulation and may lead to the development of novel strategies to modulate the immune system. Moreover, due to its role in inflammatory responses, G-CSFR has been studied in the context of autoimmune diseases, where its modulation could offer new avenues for treatment. The generation of recombinant G-CSFR/CD114 proteins allows researchers to investigate the receptor's biology in controlled settings, paving the way for both basic and translational research aimed at leveraging G-CSF signaling for therapeutic benefits. This recombinant technology facilitates assays that can elucidate receptor-ligand interactions, signaling pathways, and the receptor's role in disease, thus enhancing our understanding of granulocyte physiology and its implications in various clinical conditions.
