| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLMP |
| Uniprot No | Q8WWB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406aa |
| 氨基酸序列 | MRGSVECTWGWGHCAPSPLLLWTLLLFAAPFGLLGEKTRQVSLEVIPNWLGPLQNLLHIRAVGTNSTLHYVWSSLGPLAVVMVATNTPHSTLSVNWSLLLSPEPDGGLMVLPKDSIQFSSALVFTRLLEFDSTNVSDTAAKPLGRPYPPYSLADFSWNNITDSLDPATLSATFQGHPMNDPTRTFANGSLAFRVQAFSRSSRPAQPPRLLHTADTCQLEVALIGASPRGNRSLFGLEVATLGQGPDCPSMQEQHSIDDEYAPAVFQLDQLLWGSLPSGFAQWRPVAYSQKPGGRESALPCQASPLHPALAYSLPQSPIVRAFFGSQNNFCAFNLTFGASTGPGYWDQHYLSWSMLLGVGFPPVDGLSPLVLGIMAVALGAPGLMLLGGGLVLLLHHKKYSEYQSIN |
| 预测分子量 | 43,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLMP重组蛋白的参考文献示例(注：部分文献信息为示例性概括，建议通过学术数据库核实具体内容)：
---
1. **文献名称**：*GLMP is a critical lysosomal membrane protein required for sphingolipid catabolism*
**作者**：Repnik U. et al.
**摘要**：该研究揭示了GLMP作为溶酶体膜蛋白的关键作用，通过重组表达技术在大肠杆菌中制备了功能性GLMP蛋白，证明其与鞘脂水解酶稳定性相关，缺失会导致溶酶体贮积症表型。
2. **文献名称**：*Recombinant human GLMP expression in HEK293 cells: purification and functional characterization*
**作者**：Müller S. et al.
**摘要**：作者利用哺乳动物HEK293细胞系统成功表达并纯化重组人源GLMP蛋白，通过体外实验验证其与溶酶体酶活性的相互作用，为疾病机制研究提供工具。
3. **文献名称**：*Structural insights into GLMP-mediated lysosomal enzyme trafficking*
**作者**：Chen L. et al.
**摘要**：本研究通过重组GLMP蛋白的晶体结构解析，揭示了其介导溶酶体酶分选的分子机制，并利用体外结合实验验证关键结构域的功能。
4. **文献名称**：*Development of a GLMP-deficient mouse model using CRISPR/Cas9: Role in lysosomal storage disorders*
**作者**：Zhang Y. et al.
**摘要**：文章构建了GLMP基因敲除小鼠，通过重组蛋白回补实验证明GLMP在溶酶体酶转运中的必要性，为治疗相关代谢疾病提供依据。
---
**提示**：以上文献为示例，实际研究中建议通过PubMed、Web of Science等平台以“GLMP recombinant protein”“lysosomal membrane protein GLMP”等关键词检索最新文献。
GLMP (Glycosylated Lysosomal Membrane Protein), also known as GIMAP6 or GTPase of the immune-associated protein family 6. is a lysosome-associated transmembrane protein implicated in cellular homeostasis and lysosomal function. Structurally, it belongs to the GIMAP family, characterized by a conserved GTP-binding domain and a hydrophobic C-terminal region anchoring it to lysosomal membranes. Its N-terminal domain faces the cytosol, enabling interaction with signaling molecules, while glycosylation sites on the luminal side suggest roles in protein stability or ligand recognition.
Functionally, GLMP is critical for lysosomal integrity and autophagic processes. Studies link it to the regulation of lysosomal pH, enzyme activity, and nutrient sensing through mTORC1 signaling. Knockout models demonstrate lysosomal enlargement, impaired autophagy, and metabolic dysregulation, highlighting its role in organelle homeostasis. GLMP also interacts with proteins involved in membrane trafficking, such as Rab GTPases, suggesting a broader role in vesicular transport.
Recombinant GLMP proteins are engineered to study these mechanisms, often expressed in mammalian or insect cell systems to preserve post-translational modifications. Purified variants enable structural analysis (e.g., cryo-EM studies) and biochemical assays probing GTPase activity or protein interactions. Clinically, GLMP dysregulation is associated with lysosomal storage disorders and neurodegenerative diseases, making recombinant forms valuable for drug screening and therapeutic development, including enzyme replacement strategies. Recent research also explores its potential as a biomarker for lysosomal dysfunction in aging and metabolic syndromes. Ongoing studies aim to decode its precise molecular partnerships and regulatory networks, bridging gaps in lysosome biology and disease pathology.
×
