| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA6 |
| Uniprot No | P23280 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-308aa |
| 氨基酸序列 | QHVSDWTYSEGALDEAHWPQHYPACGGQRQSPINLQRTKVRYNPSLKGLNMTGYETQAGEFPMVNNGHTVQISLPSTMRMTVADGTVYIAQQMHFHWGGASSEISGSEHTVDGIRHVIEIHIVHYNSKYKSYDIAQDAPDGLAVLAAFVEVKNYPENTYYSNFISHLANIKYPGQRTTLTGLDVQDMLPRNLQHYYTYHGSLTTPPCTENVHWFVLADFVKLSRTQVWKLENSLLDHRNKTIHNDYRRTQPLNHRVVESNFPNQEYTLGSEFQFYLHKIEEILDYLRRALN |
| 预测分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA6(碳酸酐酶VI)重组蛋白的3篇示例参考文献(内容为模拟示例，建议通过学术数据库验证准确性)：
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1. **文献名称**: *"Expression and Purification of Recombinant Human Carbonic Anhydrase VI in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 本研究描述了一种利用大肠杆菌表达系统高效表达重组人CA6蛋白的方法，并通过亲和层析纯化获得高纯度蛋白，验证了其酶活性及稳定性，为后续功能研究奠定基础。
2. **文献名称**: *"Role of Recombinant CA6 in Salivary pH Regulation and Oral Microbial Defense"*
**作者**: Lee JH, et al.
**摘要**: 文章探讨了重组CA6蛋白在体外调节唾液pH的能力及其对口腔致病菌生长的抑制作用，提示其在口腔疾病治疗中的潜在应用价值。
3. **文献名称**: *"Development of a CA6-Specific Monoclonal Antibody Using Recombinant Protein as Antigen"*
**作者**: Zhang Y, et al.
**摘要**: 研究利用重组CA6蛋白作为免疫原成功制备了高特异性单克隆抗体，并建立了基于该抗体的ELISA检测方法，可用于唾液或血清中CA6水平的定量分析。
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**注**：以上文献信息为示例，实际文献需通过PubMed、Google Scholar等平台检索确认。CA6相关研究可关注关键词“CA6 recombinant protein”、“carbonic anhydrase VI expression”或结合具体应用领域(如癌症、口腔生物学)进行筛选。
CA6 (Carbonic Anhydrase VI), also known as gustin, is a zinc-containing glycoprotein enzyme belonging to the carbonic anhydrase family. It is primarily secreted by salivary glands, particularly the serous acinar cells of the parotid and submandibular glands, and is present in saliva. Unlike other carbonic anhydrase isoforms, CA6 is uniquely involved in oral physiological processes, including taste perception, dental health, and pH regulation in the oral cavity. It catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, contributing to the maintenance of ionic balance and enamel remineralization. CA6 has also been implicated in taste bud development and sour taste sensitivity due to its role in zinc signaling.
Recombinant CA6 protein is produced using biotechnological platforms, such as bacterial (e.g., *E. coli*) or mammalian expression systems, to enable large-scale purification for research and therapeutic applications. Its recombinant form retains enzymatic activity and structural stability, making it valuable for functional studies. Researchers utilize CA6 recombinant protein to investigate its interactions with oral pathogens, such as *Streptococcus mutans*, and its potential as a biomarker for salivary gland dysfunction, oral cancers, or systemic diseases like Sjögren’s syndrome. Additionally, CA6’s involvement in metabolic pathways has sparked interest in metabolic disorder research.
Recent studies explore CA6’s diagnostic utility, as altered CA6 levels in saliva correlate with conditions like breast cancer and cystic fibrosis. Its recombinant variant also serves as a tool for developing enzyme replacement therapies or oral care products targeting pH imbalance. Despite progress, mechanisms linking CA6 polymorphisms to taste dysfunction or caries susceptibility remain under investigation. Overall, recombinant CA6 protein bridges basic science and clinical applications, offering insights into oral-systemic health connections.
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