| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCRN4L |
| Uniprot No | Q9UK39 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MFHSPRRLCS ALLQRDAPGL RRLPAPGLRR PLSPPAAVPR PASPRLLAAA SAASGAARSC SRTVCSMGTG TSRLYSALAK TLNSSAASQH PEYLVSPDPE HLEPIDPKEL LEECRAVLHT RPPRFQRDFV DLRTDCPSTH PPIRVMQWNI LAQALGEGKD NFVQCPVEAL KWEERKCLIL EEILAYQPDI LCLQEVDHYF DTFQPLLSRL GYQGTFFPKP WSPCLDVEHN NGPDGCALFF LQNRFKLVNS ANIRLTAMTL KTNQVAIAQT LECKESGRQF CIAVTHLKAR TGWERFRSAQ GCDLLQNLQN ITQGAKIPLI VCGDFNAEPT EEVYKHFASS SLNLNSAYKL LSADGQSEPP YTTWKIRTSG ECRHTLDYIW YSKHALNVRS ALDLLTEEQI GPNRLPSFNY PSDHLSLVCD FSFTEESDGL S |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCRN4L重组蛋白的3条虚构参考文献示例(注：实际文献可能需要通过学术数据库验证)：
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1. **文献名称**：*"Recombinant CCRN4L Protein Suppresses Tumor Growth by Modulating NOTCH Signaling"*
**作者**：Wang, Y., et al.
**摘要**：本研究成功在HEK293细胞中表达了重组CCRN4L蛋白，并证明其通过抑制NOTCH信号通路抑制体外肿瘤细胞增殖和小鼠移植瘤模型中的肿瘤生长，提示其潜在抗癌作用。
2. **文献名称**：*"Structural and Functional Analysis of CCRN4L in mRNA Deadenylation Complexes"*
**作者**：Li, X., & Tanaka, K.
**摘要**：通过昆虫细胞系统表达并纯化重组CCRN4L，结合冷冻电镜结构解析，揭示其与CCR4-NOT复合体的相互作用机制，阐明其在mRNA去腺苷酸化中的关键功能。
3. **文献名称**：*"A Novel Affinity Purification Strategy for High-Efficiency Production of Recombinant CCRN4L"*
**作者**：Gupta, R., et al.
**摘要**：开发了一种基于His/SUMO双标签的重组CCRN4L纯化方法，显著提高蛋白产量和稳定性，为后续生化研究提供可靠工具。
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**提示**：若需真实文献，建议通过PubMed或Google Scholar检索“CCRN4L recombinant protein”或“CCRN4L expression”，并筛选涉及重组表达、功能或应用的论文。
CCRN4L (CCR4-NOT transcription complex subunit 4-like), also known as CNOT4L or NOT4. is a conserved eukaryotic protein involved in post-transcriptional gene regulation. It belongs to the CCR4-NOT complex, a multi-subunit assembly critical for mRNA degradation, transcription elongation, and translational control. Structurally, CCRN4L contains a RING finger domain characteristic of E3 ubiquitin ligases, enabling its role in protein ubiquitination and proteasomal targeting. Evolutionarily, its homologs are found from yeast (NOT4) to mammals, underscoring its functional significance.
Recombinant CCRN4L protein is engineered for biochemical and functional studies, typically expressed in bacterial (E. coli) or eukaryotic systems (e.g., HEK293 cells) with affinity tags like GST or His for purification. This engineered protein allows researchers to investigate its dual functionality: 1) modulating mRNA stability through CCR4-NOT complex interactions, and 2) mediating protein quality control via ubiquitination mechanisms. Studies using recombinant CCRN4L have revealed its involvement in cellular stress responses, cell cycle regulation, and apoptosis. Dysregulation of CCRN4L has been implicated in neurological disorders and cancer progression, making it a potential therapeutic target.
Current research focuses on characterizing its structural dynamics, substrate specificity, and regulatory networks. The recombinant protein serves as a tool for identifying binding partners, mapping post-translational modifications, and screening small-molecule inhibitors. Challenges persist in recapitulating its native conformational states due to the CCR4-NOT complex's size (>1 MDa) and context-dependent interactions. Nevertheless, recombinant CCRN4L remains pivotal for deciphering its role in coupling transcriptional outputs to protein homeostasis.
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