| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PI16 |
| Uniprot No | Q6UXB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-442aa |
| 氨基酸序列 | LTDEEKRLMV ELHNLYRAQV SPPASDMLHM RWDEELAAFA KAYARQCVWG HNKERGRRGE NLFAITDEGM DVPLAMEEWH HEREHYNLSA ATCSPGQMCG HYTQVVWAKT ERIGCGSHFC EKLQGVEETN IELLVCNYEP PGNVKGKRPY QEGTPCSQCP SGYHCKNSLC EPIGSPEDAQ DLPYLVTEAP SFRATEASDS RKMGTPSSLA TGIPAFLVTE VSGSLATKAL PAVETQAPTS LATKDPPSMA TEAPPCVTTE VPSILAAHSL PSLDEEPVTF PKSTHVPIPK SADKVTDKTK VPSRSPENSL DPKMSLTGAR ELLPHAQEEA EAEAELPPSS EVLASVFPAQ DKPGELQATL DHTGHTSSKS LPNFPNTSAT ANATGGRALA LQSSLPGAEG PDKPSVVSGL NSGPGAAADY KDDDDK |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PI16重组蛋白的参考文献示例(注：内容为模拟概括，建议通过学术数据库核实真实文献)：
1. **《重组PI16蛋白抑制肝星状细胞活化的分子机制研究》**
- 作者：张某某等
- 摘要：研究通过体外表达PI16重组蛋白，证实其可通过阻断TGF-β/Smad信号通路抑制肝星状细胞活化，为抗肝纤维化治疗提供潜在策略。
2. **《人源PI16重组蛋白的高效表达与功能鉴定》**
- 作者：李某某等
- 摘要：利用大肠杆菌系统成功表达并纯化人源PI16重组蛋白，发现其能特异性抑制丝氨酸蛋白酶活性，并参与细胞外基质调控。
3. **《PI16重组蛋白通过调控Wnt通路促进干细胞成骨分化》**
- 作者：王某某等
- 摘要：实验表明，PI16重组蛋白通过激活Wnt/β-catenin信号通路增强间充质干细胞的成骨分化能力，提示其在骨组织工程中的应用价值。
4. **《PI16重组蛋白在胰腺癌模型中的抗肿瘤作用》**
- 作者：陈某某等
- 摘要：动物实验证明，注射PI16重组蛋白可显著抑制胰腺癌生长，其机制涉及诱导肿瘤细胞凋亡及抑制血管内皮生长因子(VEGF)表达。
建议通过PubMed、Google Scholar等平台以“PI16 recombinant protein”、“PI16 function”等关键词检索最新文献以获取准确信息。
**Background of PI16 Recombinant Protein**
PI16 (Peptidase Inhibitor 16) is a protein encoded by the *PI16* gene, belonging to the protease inhibitor family. It is characterized by a conserved CAP domain (cysteine-rich secretory protein, antigen 5. and pathogenesis-related 1 protein), which is implicated in protein-protein interactions and regulatory functions. PI16 is predominantly expressed in tissues such as the testis, brain, and vascular smooth muscle, suggesting roles in cellular homeostasis, tissue remodeling, and immune modulation.
Recombinant PI16 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, ensuring high purity and bioactivity. Its production enables detailed study of PI16's molecular mechanisms, including its potential as a protease inhibitor and its involvement in extracellular matrix (ECM) regulation. Studies indicate that PI16 may interact with proteins like ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs), influencing ECM degradation and tissue repair processes.
Emerging research links PI16 to pathological conditions, including fibrosis, cancer, and cardiovascular diseases. For instance, elevated PI16 levels are observed in fibrotic tissues, where it may modulate fibroblast activation and collagen deposition. In cancer, PI16's role remains controversial, with evidence suggesting both tumor-suppressive and pro-metastatic effects depending on context. Additionally, PI16 has been explored as a biomarker for vascular aging and atherosclerosis due to its association with smooth muscle cell behavior.
The development of recombinant PI16 facilitates functional studies, antibody production, and therapeutic exploration. However, its precise biological pathways and clinical relevance require further investigation. Current research focuses on elucidating its dual roles in homeostasis and disease, aiming to harness its regulatory potential for targeted therapies.
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