| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CMPK1 |
| Uniprot No | P30085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLSRCRSGLL HVLGLSFLLQ TRRPILLCSP RLMKPLVVFV LGGPGAGKGT QCARIVEKYG YTHLSAGELL RDERKNPDSQ YGELIEKYIK EGKIVPVEIT ISLLKREMDQ TMAANAQKNK FLIDGFPRNQ DNLQGWNKTM DGKADVSFVL FFDCNNEICI ERCLERGKSS GRSDDNRESL EKRIQTYLQS TKPIIDLYEE MGKVKKIDAS KSVDEVFDEV VQIFDKEG |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CMPK1重组蛋白的3篇参考文献的简要总结：
1. **文献名称**：Structural and functional characterization of human CMPK1
**作者**：Li Y, et al.
**摘要**：该研究通过重组表达纯化人源CMPK1蛋白，解析其晶体结构，揭示了其底物结合和催化机制，为靶向CMPK1的药物设计提供了结构基础。
2. **文献名称**：CMPK1 regulates antiviral innate immunity by promoting mitochondrial antiviral signaling protein aggregation
**作者**：Wang R, et al.
**摘要**：研究利用重组CMPK1蛋白进行体外酶活实验，发现其通过调控MAVS蛋白的聚集增强抗病毒免疫反应，揭示了CMPK1在先天免疫中的新功能。
3. **文献名称**：Mitochondrial CMPK1 mediates metabolic adaptation in hepatocellular carcinoma
**作者**：Chen L, et al.
**摘要**：通过重组蛋白技术验证CMPK1的酶活性，证明其在肝癌细胞线粒体能量代谢重编程中的作用，为肝癌治疗提供潜在靶点。
注：以上文献信息为示例，实际文献需通过PubMed或相关数据库检索确认。若需具体文献，建议使用关键词“CMPK1 recombinant”“CMPK1 purification”进一步筛选。
**Background of CMPK1 Recombinant Protein**
CMPK1 (cytidine/uridine monophosphate kinase 1) is a cytoplasmic nucleotide kinase that plays a critical role in nucleotide metabolism. It catalyzes the phosphorylation of cytidine monophosphate (CMP) and uridine monophosphate (UMP) to their diphosphate forms (CDP and UDP), which are essential precursors for DNA/RNA synthesis and glycosylation processes. CMPK1 is ubiquitously expressed and regulates cellular proliferation, mitochondrial function, and energy homeostasis by maintaining nucleotide pools. Dysregulation of CMPK1 has been linked to mitochondrial disorders, viral replication, and cancer progression, highlighting its biological and therapeutic relevance.
Recombinant CMPK1 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian systems, followed by purification to ensure high homogeneity and activity. This engineered protein retains the enzymatic properties of native CMPK1. enabling researchers to study its kinetic mechanisms, substrate specificity, and interactions with inhibitors or regulatory molecules. Its recombinant form is widely used in *in vitro* assays to investigate nucleotide metabolism pathways, screen antiviral or anticancer drugs targeting nucleotide biosynthesis, and explore molecular mechanisms underlying diseases like mitochondrial myopathies or viral infections.
Structurally, recombinant CMPK1 often includes affinity tags (e.g., His-tag) for simplified purification and detection. Studies utilizing this protein have revealed insights into its ATP-binding domain, catalytic residues, and conformational changes during substrate binding. Additionally, CMPK1's role in supporting viral replication (e.g., HCV, HIV) by providing nucleotide substrates has spurred interest in developing host-targeted antiviral therapies.
Overall, CMPK1 recombinant protein serves as a vital tool for both basic research and drug discovery, bridging the understanding of nucleotide metabolism with therapeutic innovation.
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