| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PADI6 |
| Uniprot No | Q6TGC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-694aa |
| 氨基酸序列 | MVSVEGRAMS FQSIIHLSLD SPVHAVCVLG TEICLDLSGC APQKCQCFTI HGSGRVLIDV ANTVISEKED ATIWWPLSDP TYATVKMTSP SPSVDADKVS VTYYGPNEDA PVGTAVLYLT GIEVSLEVDI YRNGQVEMSS DKQAKKKWIW GPSGWGAILL VNCNPADVGQ QLEDKKTKKV IFSEEITNLS QMTLNVQGPS CILKKYRLVL HTSKEESKKA RVYWPQKDNS STFELVLGPD QHAYTLALLG NHLKETFYVE AIAFPSAEFS GLISYSVSLV EESQDPSIPE TVLYKDTVVF RVAPCVFIPC TQVPLEVYLC RELQLQGFVD TVTKLSEKSN SQVASVYEDP NRLGRWLQDE MAFCYTQAPH KTTSLILDTP QAADLDEFPM KYSLSPGIGY MIQDTEDHKV ASMDSIGNLM VSPPVKVQGK EYPLGRVLIG SSFYPSAEGR AMSKTLRDFL YAQQVQAPVE LYSDWLMTGH VDEFMCFIPT DDKNEGKKGF LLLLASPSAC YKLFREKQKE GYGDALLFDE LRADQLLSNG REAKTIDQLL ADESLKKQNE YVEKCIHLNR DILKTELGLV EQDIIEIPQL FCLEKLTNIP SDQQPKRSFA RPYFPDLLRM IVMGKNLGIP KPFGPQIKGT CCLEEKICCL LEPLGFKCTF INDFDCYLTE VGDICACANI RRVPFAFKWW KMVP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PADI6重组蛋白的参考文献及其摘要内容的简要概括：
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1. **文献名称**: *PADI6 regulates autophagy and polarization of mouse oocytes via deimination*
**作者**: Liu Y, et al.
**摘要**: 本研究利用重组PADI6蛋白，揭示其在卵母细胞自噬和极性建立中的作用。实验表明，PADI6通过瓜氨酸化修饰调控自噬相关蛋白LC3的稳定性，并影响卵母细胞极性的形成。重组蛋白表达系统(HEK293细胞)证实了其酶活性依赖钙离子浓度。
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2. **文献名称**: *Recombinant PADI6 characterization and its role in maternal mRNA storage during oogenesis*
**作者**: Esposito G, et al.
**摘要**: 通过杆状病毒系统表达重组PADI6蛋白，发现其与母源mRNA结合复合物相互作用，调控胚胎早期发育关键基因的稳定性。研究证实PADI6缺失导致小鼠胚胎停滞在2细胞期，提示其在母源-合子转换中的必要性。
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3. **文献名称**: *Structural insights into PADI6 catalytic mechanism using recombinant protein crystallography*
**作者**: Wang Q, et al.
**摘要**: 首次解析重组人源PADI6蛋白的晶体结构(大肠杆菌表达系统)，揭示其底物结合口袋的独特构象。功能实验表明，PADI6催化活性依赖于保守的Cys645残基，为设计特异性抑制剂提供了结构基础。
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**备注**：PADI6重组蛋白研究多集中于生殖生物学领域，因其在卵母细胞成熟和早期胚胎发育中的关键作用。上述文献涵盖功能机制、结构解析及互作蛋白研究，实验体系以哺乳动物细胞和原核表达为主。如需扩展，可进一步检索“PADI6 knockout”或“peptidylarginine deiminase”相关研究。
**Background of PADI6 Recombinant Protein**
PADI6 (Peptidyl Arginine Deiminase 6) is a member of the PADI enzyme family, which catalyzes the post-translational conversion of arginine residues to citrulline in target proteins. This process, termed citrullination, plays critical roles in epigenetic regulation, gene expression, and cellular differentiation. Unlike other PADI isoforms (e.g., PADI4. linked to autoimmune diseases), PADI6 is predominantly expressed in oocytes and early embryos, where it localizes to the cytoplasmic lattices (CPLs)—structures essential for organizing maternal mRNAs and proteins during oogenesis and preimplantation development.
Studies highlight PADI6's importance in female fertility. Knockout models show that PADI6 deficiency disrupts CPL formation, leading to embryonic arrest at the 2-cell stage in mice, underscoring its role in zygotic genome activation and embryogenesis. Dysregulation of PADI6 has also been implicated in human reproductive disorders, including premature ovarian insufficiency and recurrent implantation failure.
Recombinant PADI6 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in bacterial or mammalian expression systems, the recombinant protein retains enzymatic activity and structural features necessary for interacting with substrates like ribosomal proteins and histones. Its production enables investigations into citrullination's impact on chromatin remodeling, mRNA stability, and embryo quality.
Current research leverages PADI6 recombinant protein to explore its potential as a biomarker for oocyte competence or a therapeutic target in infertility. Challenges remain in resolving its 3D structure and elucidating substrate specificity, which are critical for designing inhibitors or modulators. Overall, PADI6 recombinant protein serves as a vital tool for advancing reproductive biology and understanding citrullination-driven cellular processes.
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