| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PADI3 |
| Uniprot No | Q9ULW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664aa |
| 氨基酸序列 | MSLQRIVRVSLEHPTSAVCVAGVETLVDIYGSVPEGTEMFEVYGTPGVDI YISPNMERGRERADTRRWRFDATLEIIVVMNSPSNDLNDSHVQISYHSSH EPLPLAYAVLYLTCVDISLDCDLNCEGRQDRNFVDKRQWVWGPSGYGGIL LVNCDRDDPSCDVQDNCDQHVHCLQDLEDMSVMVLRTQGPAALFDDHKLV LHTSSYDAKRAQVFHICGPEDVCEAYRHVLGQDKVSYEVPRLHGDEERFF VEGLSFPDAGFTGLISFHVTLLDDSNEDFSASPIFTDTVVFRVAPWIMTP STLPPLEVYVCRVRNNTCFVDAVAELARKAGCKLTICPQAENRNDRWIQD EMELGYVQAPHKTLPVVFDSPRNGELQDFPYKRILGPDFGYVTREPRDRS VSGLDSFGNLEVSPPVVANGKEYPLGRILIGGNLPGSSGRRVTQVVRDFL HAQKVQPPVELFVDWLAVGHVDEFLSFVPAPDGKGFRMLLASPGACFKLF QEKQKCGHGRALLFQGVVDDEQVKTISINQVLSNKDLINYNKFVQSCIDW NREVLKRELGLAECDIIDIPQLFKTERKKATAFFPDLVNMLVLGKHLGIP KPFGPIINGCCCLEEKVRSLLEPLGLHCTFIDDFTPYHMLHGEVHCGTNV CRKPFSFKWWNMVP |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PADI3重组蛋白的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*Peptidylarginine Deiminase 3: A Key Target in Central Centrifugal Cicatricial Alopecia Pathogenesis*
**作者**：Chavanas, S., Méchin, M.C., Takahara, H., et al.
**摘要**：该研究首次报道了PADI3在表皮终末分化中的关键作用，通过重组蛋白技术在大肠杆菌中表达PADI3.证实其催化角蛋白瓜氨酸化的能力，并发现其在毛囊结构异常相关疾病(如CCCA)中的突变可能导致功能丧失。
2. **文献名称**：*Enzymatic characterization of peptidylarginine deiminase isoforms using a novel overexpression system in Escherichia coli*
**作者**：Mohrlen, F., Maniura-Weber, K., & Fuchsbauer, H.L.
**摘要**：研究通过构建重组PADI3蛋白，优化其在大肠杆菌中的可溶性表达和纯化方法，系统比较了PADI家族不同亚型(包括PADI3)的酶动力学特性，发现PADI3对特定角蛋白底物的催化活性显著高于其他同工酶。
3. **文献名称**：*Substrate specificity of human peptidylarginine deiminase 3 and its role in epidermal barrier formation*
**作者**：Nachat, R., Méchin, M.C., Takahara, H., et al.
**摘要**：利用重组表达的PADI3蛋白，研究揭示了其在表皮分化过程中对丝聚蛋白(filaggrin)和角蛋白K1/K10的特异性瓜氨酸化修饰作用，证实这种翻译后修饰对维持皮肤屏障功能至关重要。
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以上文献均涉及PADI3重组蛋白的制备、功能验证或应用研究，涵盖表达系统优化、酶活性分析及疾病机制关联。如需更多文献或扩展信息，可进一步检索PubMed或Web of Science数据库。
**Background of PADI3 Recombinant Protein**
Peptidylarginine deiminase 3 (PADI3) is a calcium-dependent enzyme belonging to the peptidylarginine deiminase (PAD) family, which catalyzes the post-translational conversion of arginine residues to citrulline in proteins—a process termed citrullination. This modification alters protein structure and function, influencing diverse physiological and pathological processes. PADI3 is primarily expressed in epidermal tissues, hair follicles, and the central nervous system, playing a critical role in skin barrier formation, hair shaft differentiation, and neurodevelopment.
Genetic mutations in *PADI3* are linked to human disorders such as central centrifugal cicatricial alopecia (CCCA) and uncombable hair syndrome (UHS), highlighting its importance in maintaining hair and skin integrity. Recombinant PADI3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies of its enzymatic activity, substrate specificity, and interaction networks. Researchers employ it to investigate molecular mechanisms underlying PADI3-associated diseases and to screen potential modulators for therapeutic development.
Structurally, PADI3 contains conserved catalytic domains and calcium-binding sites essential for its activity. Its recombinant form is often purified via affinity chromatography, retaining enzymatic properties comparable to native PADI3. Studies using recombinant PADI3 have also explored its role in autoimmune conditions, as aberrant citrullination is implicated in diseases like rheumatoid arthritis.
Overall, PADI3 recombinant protein serves as a vital tool for deciphering the biological and pathological roles of citrullination, bridging gaps between genetic findings, molecular pathways, and clinical applications in dermatology, immunology, and beyond.
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