| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PADI2 |
| Uniprot No | Q9Y2J8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665aa |
| 氨基酸序列 | MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWV EVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDE EGSIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASWTWGPEGQGAI LLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEI VLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLF FVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMT PNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRW IQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPL FESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPLSGGRRMTKVVR DFLKAQQVQAPVELYSDWLTVGHVDEFMSFVPIPGTKKFLLLMASTSACY KLFREKQKDGHGEAIMFKGLGGMSSKRITINKILSNESLVQENLYFQRCL DWNRDILKKELGLTEQDIIDLPALFKMDEDHRARAFFPNMVNMIVLDKDL GI PKPFGPQVEEECCLEMHVRGLLEPLGLECTFIDDISAYHKFLGEVH CGTNVRRKPFTFKWWHMVP |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PADI2重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用请核实真实来源)：
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1. **文献名称**: *Expression and characterization of recombinant human PADI2 in Escherichia coli*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达了具有活性的重组人源PADI2蛋白，并通过优化表达条件和纯化步骤(如镍柱亲和层析)获得高纯度蛋白。酶活性分析表明，重组PADI2可有效催化组蛋白H3的瓜氨酸化，为后续功能研究提供了工具。
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2. **文献名称**: *Structural insights into PADI2 activation by calcium ions via recombinant protein crystallography*
**作者**: Lee B, et al.
**摘要**: 通过重组表达并纯化PADI2蛋白，结合X射线晶体学技术解析了其与钙离子结合的活性结构，揭示了PADI2依赖钙离子激活的分子机制，为设计靶向PADI2的抑制剂奠定了基础。
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3. **文献名称**: *Recombinant PADI2 mediates citrullination of extracellular matrix proteins in rheumatoid arthritis*
**作者**: Chen Y, et al.
**摘要**: 研究利用哺乳动物细胞表达的重组PADI2蛋白，证实其能够在体外催化纤维蛋白原和波形蛋白的瓜氨酸化，提示PADI2可能通过修饰细胞外基质蛋白参与类风湿性关节炎的发病机制。
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(注：以上文献信息为模拟生成，实际研究中请查阅PubMed、Web of Science等数据库获取真实文献。)
**Background of PADI2 Recombinant Protein**
Peptidyl arginine deiminase 2 (PADI2) belongs to the PADI enzyme family, which catalyzes the post-translational conversion of arginine residues to citrulline in proteins. This process, termed citrullination or deimination, alters protein structure and function, influencing diverse physiological and pathological processes. PADI2 is widely expressed in various tissues, including the brain, immune system, and reproductive organs, and plays roles in gene regulation, inflammation, and cellular differentiation.
Research has linked PADI2 dysregulation to diseases such as rheumatoid arthritis, multiple sclerosis, and cancers. For instance, aberrant citrullination of histones or extracellular matrix proteins by PADI2 may contribute to autoimmune responses or tumor progression. Its involvement in epigenetic modulation—via histone citrullination affecting chromatin structure—highlights its importance in transcriptional regulation.
Recombinant PADI2 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purified PADI2 retains enzymatic activity, allowing researchers to investigate substrate specificity, catalytic mechanisms, and inhibitor interactions. It is instrumental in developing assays for drug discovery, exploring disease mechanisms, and generating citrulline-specific antibodies.
Studies using recombinant PADI2 have advanced understanding of its role in cellular stress responses, neural development, and immune tolerance. Its application in structural biology (e.g., crystallography) has also provided insights into enzyme dynamics and active-site targeting. As citrullination gains recognition in disease pathways, recombinant PADI2 remains a critical tool for unraveling its biological significance and therapeutic potential.
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