| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALPPL2 |
| Uniprot No | P10696 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-503aa |
| 氨基酸序列 | I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG TTD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALPPL2重组蛋白的3篇假设参考文献及其摘要概括，供参考：
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1. **文献名称**：ALPPL2 as a Novel Tumor-Specific Antigen in Germ Cell Tumors
**作者**：Hosen N. et al.
**摘要**：研究证实ALPPL2在睾丸癌、卵巢癌等生殖细胞肿瘤中特异性高表达，而正常组织几乎不表达。通过大肠杆菌系统成功表达ALPPL2重组蛋白，并验证其作为血清学标志物和免疫治疗靶点的潜力。
2. **文献名称**：Recombinant ALPPL2 Protein for CAR-T Cell Therapy Targeting Solid Tumors
**作者**：Tran T.T. et al.
**摘要**：利用HEK293细胞表达ALPPL2重组蛋白，筛选出高亲和力抗体并构建CAR-T细胞。实验显示，该重组蛋白诱导的CAR-T对ALPPL2阳性肿瘤细胞具有显著杀伤活性，为实体瘤免疫治疗提供新策略。
3. **文献名称**：Structural and Functional Analysis of ALPPL2 via Recombinant Protein Crystallography
**作者**：Hammo Y. et al.
**摘要**：通过昆虫杆状病毒系统表达并纯化ALPPL2重组蛋白，解析其三维晶体结构，揭示其底物结合域的关键氨基酸位点，为开发靶向抑制剂奠定结构基础。
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*注：以上文献为基于公开领域研究的假设性示例，实际引用时需以真实发表的论文为准。建议通过PubMed或Web of Science等平台检索最新文献。*
**Background of ALPPL2 Recombinant Protein**
ALPPL2 (Alkaline Phosphatase, Placental-Like 2), also known as germ cell alkaline phosphatase (GCAP), is a glycosylphosphatidylinositol (GPI)-anchored membrane protein belonging to the alkaline phosphatase family. It shares structural and functional homology with placental alkaline phosphatase (ALPP) but is distinguished by its tissue-specific expression. ALPPL2 is primarily expressed in germ cells, certain cancers (e.g., testicular and ovarian cancers), and during early embryonic development. Its physiological role remains unclear, though it is hypothesized to participate in phosphate metabolism, cell differentiation, or immune modulation.
Recombinant ALPPL2 protein is engineered using biotechnological methods, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation, which are critical for its stability and function. The recombinant form retains the enzymatic activity of native ALPPL2. enabling its use in functional studies.
ALPPL2 has garnered attention as a tumor-associated antigen due to its overexpression in malignancies, particularly germ cell tumors and some somatic cancers. This makes it a potential biomarker for cancer diagnosis and a target for immunotherapy. For example, ALPPL2-specific antibodies or CAR-T cells are being explored for targeted cancer therapies. Additionally, recombinant ALPPL2 is utilized in immunoassays to detect autoantibodies in autoimmune disorders or to study its interaction with biological molecules.
Despite its promise, research on ALPPL2 is ongoing to fully elucidate its biological significance and therapeutic potential. Its restricted normal tissue expression reduces the risk of off-target effects in treatments, highlighting its translational value in precision medicine.
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