| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPZ |
| Uniprot No | Q66K79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652aa |
| 氨基酸序列 | MPPPLPLLLLTVLVVAAARPGCEFERNPAGECHRPPAADSATCVDLQLRTCSDAAYNHTTFPNLLQHRSWEVVEASSEYILLSVLHQLLEGQCNPDLRLLGCAVLAPRCEGGWVRRPCRHICEGLREVCQPAFDAIDMAWPYFLDCHRYFTREDEGCYDPLEKLRGGLEADEALPSGLPPTFIRFSHHSYAQMVRVLRRTASRCAHVARTYSIGRSFDGRELLVIEFSSRPGQHELMEPEVKLIGNIHGNEVAGREMLIYLAQYLCSEYLLGNPRIQRLLNTTRIHLLPSMNPDGYEVAAAEGAGYNGWTSGRQNAQNLDLNRNFPDLTSEYYRLAETRGARSDHIPIPQHYWWGKVAPETKAIMKWMQTIPFVLSASLHGGDLVVSYPFDFSKHPQEEKMFSPTPDEKMFKLLSRAYADVHPMMMDRSENRCGGNFLKRGSIINGADWYSFTGGMSDFNYLHTNCFEITVELGCVKFPPEEALYILWQHNKESLLNFVETVHRGIKGVVTDKFGKPVKNARISVKGIRHDITTAPDGDYWRLLPPGIHIVIAQAPGYAKVIKKVIIPARMKRAGRVDFILQPLGMGPKNFIHGLRRTGPHDPLGGASSLGEATEPDPLRARRQPSADGSKPWWWSYFTSLSTHRPRWLLKY |
| 预测分子量 | 73,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CPZ(假设为某种重组蛋白)的虚构参考文献示例，供参考格式和内容(实际文献请通过学术数据库查询)：
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1. **标题**：*High-yield production of recombinant CPZ protein in E. coli: Optimization and purification*
**作者**：Smith A, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效生产CPZ重组蛋白的优化策略，采用亲和层析和尺寸排阻色谱纯化，获得了高纯度且具有生物活性的CPZ蛋白，适用于体外功能研究。
2. **标题**：*Structural characterization of CPZ using X-ray crystallography*
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析了CPZ重组蛋白的三维结构，揭示了其活性位点的关键氨基酸残基，为基于结构的药物设计提供了分子基础。
3. **标题**：*Functional analysis of CPZ in neuronal apoptosis signaling pathways*
**作者**：Garcia R, et al.
**摘要**：利用重组CPZ蛋白研究其在神经元凋亡中的作用，发现CPZ通过调控线粒体膜电位和caspase-3激活参与细胞死亡信号通路。
4. **标题**：*Development of a CPZ-specific monoclonal antibody using recombinant protein as antigen*
**作者**：Wang Y, et al.
**摘要**：以纯化的重组CPZ蛋白为抗原，成功制备了高特异性的单克隆抗体，并验证了其在免疫组化和Western blot中的应用潜力。
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**备注**：
- 以上文献为虚构示例，实际研究请检索PubMed、Web of Science等数据库，使用关键词如“recombinant CPZ protein”、“CPZ expression”、“CPZ functional study”等。
- 若CPZ指代特定蛋白(如羧肽酶Z、钙蛋白酶等)，建议补充全称以精准检索。
**Background of CPZ Recombinant Protein**
Carboxypeptidase Z (CPZ), a member of the metallocarboxypeptidase family, is a zinc-dependent enzyme involved in proteolytic processing and regulatory pathways. Structurally, CPZ contains a thrombospondin type 1 (TSP1) domain and a catalytic domain, enabling its role in substrate recognition and enzymatic cleavage of C-terminal amino acids, particularly basic residues like arginine. It is implicated in diverse biological processes, including embryonic development, tissue remodeling, and signaling modulation, notably within the Wnt pathway.
CPZ is expressed in various tissues, with elevated levels observed in the liver, kidney, and brain. Its function extends beyond proteolysis; it regulates extracellular matrix dynamics by interacting with growth factors and modulating their activity. Dysregulation of CPZ has been associated with pathological conditions such as fibrosis, cancer metastasis, and neurological disorders, highlighting its therapeutic potential.
Recombinant CPZ protein is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications. The purified protein retains enzymatic activity and structural integrity, making it valuable for *in vitro* studies, including substrate specificity assays, inhibitor screening, and mechanistic investigations of Wnt signaling.
Research on CPZ recombinant protein also explores its utility in drug development. For instance, inhibitors targeting CPZ’s catalytic site could attenuate fibrosis, while engineered variants might enhance tissue repair. Additionally, CPZ’s role in processing bioactive peptides positions it as a biomarker or therapeutic target in diseases with disrupted proteostasis.
Overall, CPZ recombinant protein serves as a critical tool for unraveling its biological functions and translational applications in biomedicine.
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