| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CES5A |
| Uniprot No | Q6NT32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-575aa |
| 氨基酸序列 | APTKGPSAEG PQRNTRLGWI QGKQVTVLGS PVPVNVFLGV PFAAPPLGSL RFTNPQPASP WDNLREATSY PNLCLQNSEW LLLDQHMLKV HYPKFGVSED CLYLNIYAPA HADTGSKLPV LVWFPGGAFK TGSASIFDGS ALAAYEDVLV VVVQYRLGIF GFFTTWDQHA PGNWAFKDQV AALSWVQKNI EFFGGDPSSV TIFGESAGAI SVSSLILSPM AKGLFHKAIM ESGVAIIPYL EAHDYEKSED LQVVAHFCGN NASDSEALLR CLRTKPSKEL LTLSQKTKSF TRVVDGAFFP NEPLDLLSQK AFKAIPSIIG VNNHECGFLL PMKEAPEILS GSNKSLALHL IQNILHIPPQ YLHLVANEYF HDKHSLTEIR DSLLDLLGDV FFVVPALITA RYHRDAGAPV YFYEFRHRPQ CFEDTKPAFV KADHADEVRF VFGGAFLKGD IVMFEGATEE EKLLSRKMMK YWATFARTGN PNGNDLSLWP AYNLTEQYLQ LDLNMSLGQR LKEPRVDFWT STIPLILSAS DMLHSPLSSL TFLSLLQPFF FFCAP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CES5A重组蛋白的3篇模拟参考文献示例(内容基于常见研究主题构造，非真实文献)：
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1. **文献名称**: "Cloning, Expression, and Characterization of Recombinant Human CES5A in *E. coli*: Implications for Drug Metabolism"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了人源CES5A蛋白，并通过亲和层析纯化获得高纯度酶。酶动力学分析表明，CES5A对紫杉醇前体(paclitaxel-7-xyloside)具有显著水解活性(Km=12 μM)，最适pH为7.5.提示其在肝脏药物代谢中的潜在作用。
2. **文献名称**: "Structural and Functional Analysis of CES5A Mutants Reveals Substrate-Specific Catalytic Mechanisms"
**作者**: Wang Y, et al.
**摘要**: 通过定点突变技术构建了CES5A的催化三联体突变体(Ser221Ala, His464Gln, Glu341Asp)，发现其水解活性丧失。分子对接模拟显示，CES5A的疏水口袋对脂溶性药物(如伊立替康)的结合效率起关键作用，为设计CES5A靶向抑制剂提供依据。
3. **文献名称**: "CES5A Overexpression Enhances the Activation of Anticancer Prodrug in Hepatocellular Carcinoma Cells"
**作者**: Li X, et al.
**摘要**: 在肝癌细胞中过表达重组CES5A蛋白，显著提高了前药卡培他滨(capecitabine)转化为5-FU的效率，导致肿瘤细胞凋亡率增加。研究证实CES5A的表达水平与肝癌患者的化疗敏感性呈正相关。
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注：以上文献为示例性内容，实际研究中请通过PubMed/Google Scholar等平台检索真实文献(关键词：CES5A, recombinant protein, carboxylesterase)。
**Background of CES5A Recombinant Protein**
Carboxylesterase 5A (CES5A), a member of the carboxylesterase (CES) enzyme family, plays a critical role in xenobiotic metabolism and lipid homeostasis. CES enzymes are serine hydrolases that catalyze the hydrolysis of ester-, thioester-, and amide-containing compounds, including clinically used drugs (e.g., antivirals, chemotherapeutics), environmental toxins, and endogenous lipids. CES5A, encoded by the *CES5A* gene (also termed *CES2* in some classifications), is primarily expressed in the liver and gastrointestinal tract, where it contributes to first-pass drug metabolism and influences pharmacokinetics.
Recombinant CES5A protein is produced via heterologous expression systems, such as *E. coli*, insect cells, or mammalian cell lines, enabling large-scale purification for functional studies. Its production often involves tagging (e.g., His-tag) to facilitate affinity chromatography. The recombinant protein retains enzymatic activity, allowing researchers to study substrate specificity, inhibition kinetics, and interactions with prodrugs or lipid substrates.
Research on CES5A is driven by its clinical relevance. Polymorphisms in *CES5A* may alter drug efficacy or toxicity, impacting individualized therapy. Additionally, CES5A is implicated in metabolic disorders, including obesity and fatty liver disease, due to its role in lipid processing. In drug development, recombinant CES5A is used to screen prodrug activation or assess metabolic stability of compounds.
Understanding CES5A’s structure-function relationships and regulatory mechanisms also aids in designing CES inhibitors or modulators for therapeutic purposes. Overall, CES5A recombinant protein serves as a vital tool in pharmacology, toxicology, and molecular biology, bridging insights into enzyme dynamics and their translational applications.
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