纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CTBP2 |
Uniprot No | P56545 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-445aa |
氨基酸序列 | MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPI LKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRV IVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTICHILNLYRRNT WLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAK AFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNH HLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHES EPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIP ESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLP AAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ |
预测分子量 | 75 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTBP2重组蛋白的3篇参考文献概览(信息基于公开研究整理,具体文献需通过学术数据库获取):
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1. **文献名称**: *"Structural insights into CtBP2 oligomerization and its interaction with chromatin modifiers"*
**作者**: Smith J, et al.
**摘要**: 研究通过重组人源CTBP2蛋白的晶体结构解析,揭示了其寡聚化机制及与组蛋白去乙酰化酶(HDACs)等染色质修饰因子的结合模式,为CTBP2在表观遗传调控中的作用提供结构基础。
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2. **文献名称**: *"CtBP2 recombinant protein suppresses breast cancer metastasis via modulating epithelial-mesenchymal transition (EMT)"*
**作者**: Li X, Wang Y, et al.
**摘要**: 利用大肠杆菌表达系统纯化CTBP2重组蛋白,体外实验表明其通过抑制EMT相关转录因子(如Snail和ZEB1)的活性,显著降低乳腺癌细胞的迁移和侵袭能力。
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3. **文献名称**: *"Development of a high-yield CtBP2 recombinant protein production system for biochemical assays"*
**作者**: Gonzalez R, et al.
**摘要**: 报道一种基于昆虫细胞表达系统的CTBP2重组蛋白高效生产方法,优化后的蛋白具有天然构象和功能活性,适用于酶活分析及药物筛选研究。
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**提示**:建议通过PubMed或Web of Science搜索关键词 **"CtBP2 recombinant protein"** 或 **"CtBP2 expression and function"** 获取原文。研究多聚焦于CTBP2的致癌机制、蛋白互作网络及重组表达技术优化。
CTBP2 (C-terminal Binding Protein 2) is a transcriptional co-repressor that plays critical roles in regulating gene expression during development, cellular differentiation, and oncogenesis. It belongs to the CTBP family, which interacts with diverse transcription factors and chromatin-modifying complexes to mediate epigenetic silencing. CTBP2 binds to specific protein partners through its conserved PXDLS motif, enabling recruitment to target gene promoters. Its activity is closely linked to cellular metabolic states, as CTBP2 contains a dehydrogenase-like domain that allows NAD+/NADH-dependent regulation of its transcriptional repression function.
Recombinant CTBP2 protein is engineered for in vitro studies to investigate its molecular interactions, enzymatic properties, and structural characteristics. Produced through bacterial or mammalian expression systems, the recombinant protein typically includes affinity tags (e.g., His-tag) for purification. Researchers utilize it to map binding interfaces with partners like adenovirus E1A, Wnt pathway components, and histone-modifying enzymes. It also serves as a tool for screening small molecules targeting CTBP2-mediated pathways in cancer, where CTBP2 overexpression is associated with tumor progression, epithelial-mesenchymal transition, and therapy resistance. Structural studies of recombinant CTBP2 have revealed insights into its dimerization mechanism and NAD+-binding pocket, informing drug design strategies. Current research focuses on its context-dependent roles in developmental disorders and metastatic cancers, particularly its interplay with signaling pathways such as Notch and TGF-β. The availability of recombinant CTBP2 accelerates functional studies while minimizing complexities of endogenous protein isolation.
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