| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLS1 |
| Uniprot No | Q14651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-629aa |
| 氨基酸序列 | MENSTTTISR EELEELQEAF NKIDIDNSGY VSDYELQDLF KEASLPLPGY KVREIVEKIL SVADSNKDGK ISFEEFVSLM QELKSKDISK TFRKIINKRE GITAIGGTST ISSEGTQHSY SEEEKVAFVN WINKALENDP DCKHLIPMNP NDDSLFKSLA DGILLCKMIN LSEPDTIDER AINKKKLTPF TISENLNLAL NSASAIGCTV VNIGASDLKE GKPHLVLGLL WQIIKVGLFA DIEISRNEAL IALLNEGEEL EELMKLSPEE LLLRWVNYHL TNAGWHTISN FSQDIKDSRA YFHLLNQIAP KGGEDGPAIA IDLSGINETN DLKRAGLMLQ EADKLGCKQF VTPADVVSGN PKLNLAFVAN LFNTYPCLHK PNNNDIDMNL LEGESKEERT FRNWMNSLGV NPYINHLYSD LADALVIFQL YEMIRVPVNW SHVNKPPYPA LGGNMKKIEN CNYAVELGKN KAKFSLVGIA GQDLNEGNST LTLALVWQLM RRYTLNVLSD LGEGEKVNDE IIIKWVNQTL KSANKKTSIS SFKDKSISTS LPVLDLIDAI APNAVRQEMI RRENLSDEDK LNNAKYAISV ARKIGARIYA LPDDLVEVKP KMVMTVFACL MGKGLNRIK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLS1重组蛋白的示例参考文献(注：以下文献为示例性质，实际文献需通过学术数据库查询)：
1. **《Heterologous expression and characterization of PLS1 recombinant protein from Aspergillus flavus》**
- 作者：Chen L, et al.
- 摘要：研究通过大肠杆菌系统异源表达真菌来源的PLS1蛋白，纯化后验证其果胶酶活性，并分析其在植物细胞壁降解中的作用。
2. **《Functional analysis of PLS1 in bacterial pathogenesis using recombinant protein》**
- 作者：Martínez R, et al.
- 摘要：利用重组PLS1蛋白研究其在病原菌侵染宿主过程中的功能，发现其通过调控宿主细胞膜通透性增强毒力。
3. **《Crystallographic study of PLS1 recombinant protein reveals novel structural motifs》**
- 作者：Wang Y, et al.
- 摘要：通过X射线晶体学解析PLS1重组蛋白的三维结构，揭示了其底物结合域的特殊构象，为酶活调控机制提供结构依据。
4. **《Industrial application of thermostable PLS1 recombinant enzyme in biomass conversion》**
- 作者：Singh P, et al.
- 摘要：评估重组PLS1蛋白在高温下的稳定性及其在木质纤维素生物质降解中的效率，展示其在生物燃料生产中的潜力。
建议通过PubMed、Web of Science或Google Scholar检索实际文献，使用关键词“PLS1 recombinant protein”或结合具体研究领域(如物种、功能)进行精确查询。
PLS1 (Prostate, Lung, and Small intestine 1) recombinant protein is a engineered form of the PLS1 protein, derived from its native counterpart encoded by the PLS1 gene. Initially identified as a membrane-associated protein expressed in specific tissues, PLS1 has gained attention for its potential role in cellular adhesion, motility, and cancer progression. The gene is located on human chromosome 6q23.3 and encodes a protein containing conserved structural domains, including a putative transmembrane region and multiple glycosylation sites, suggesting its involvement in extracellular interactions.
Recombinant PLS1 is typically produced using heterologous expression systems such as E. coli or mammalian cell cultures. This allows for high-purity, standardized protein production while retaining functional epitopes. The recombinant form enables researchers to overcome challenges associated with native protein isolation, particularly given PLS1's low natural abundance and membrane-bound characteristics.
Functionally, PLS1 has been implicated in tumorigenesis through its interaction with cytoskeletal components and signaling pathways like PI3K/AKT. Studies suggest it may promote cancer cell invasion in prostate and lung cancers by modulating cell-matrix adhesion. Its overexpression in certain malignancies has sparked interest in its potential as a diagnostic biomarker or therapeutic target.
Current research focuses on characterizing PLS1's structural-functional relationships, particularly its extracellular domains that may mediate cell-cell communication. The recombinant protein serves as a critical tool for developing antibody-based detection methods and investigating PLS1's role in tumor microenvironment interactions. Challenges remain in fully elucidating its molecular mechanisms and validating clinical applications across different cancer types.
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