| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K6 |
| Uniprot No | O95382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1288aa |
| 氨基酸序列 | MAGPCPRSGAERAGSCWQDPLAVALSRGRQLAAPPGRGCARSRPLSVVYVLTREPQPGLEPREGTEAEPLPLRCLREACAQVPRPRPPPQLRSLPFGTLELGDTAALDAFYNADVVVLEVSSSLVQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGLADGLVQAGVGTEALLTPLVGRLARLLEATPTDSCGYFRETIRRDIRQARERFSGPQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDRAKALSVLLPLVQLEGSVAPDLYCMCGRIYKDMFFSSGFQDAGHREQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSKELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDPTQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPPGGPPRRAHFWLHFLLQSCQPFKTACAQGDQCLVLVLEMNKVLLPAKLEVRGTDPVSTVTLSLLEPETQDIPSSWTFPVASICGVSASKRDERCCFLYALPPAQDVQLCFPSVGHCQWFCGLIQAWVTNPDSTAPAEEAEGAGEMLEFDYEYTETGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHRRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLGYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLLGDPFLQPGKRSRSPSSPRHAPRPSDAPSASPTPSANSTTQSQTFPCPQAPSQHPPSPPKRCLSYGGTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQELPALAENLHQEQKQEQGARLGRNHVEELLRCLGAHIHTPNRRQLAQELRALQGRLRAQGLGPALLHRPLFAFPDAVKQILRKRQIRPHWMFVLDSLLSRAVRAALGVLGPEVEKEAVSPRSEELSNEGDSQQSPGQQSPLPVEPEQGPAPLMVQLSLLRAETDRLREILAGKEREYQALVQRALQRLNEEARTYVLAPEPPTALSTDQGLVQWLQELNVDSGTIQMLLNHSFTLHTLLTYATRDDLIYTRIRGGMVCRIWRAILAQRAGSTPVTSGP |
| 预测分子量 | 142,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP3K6(MEKK6)重组蛋白的3篇代表性文献概览(基于领域知识综合，建议通过学术数据库验证具体信息)：
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1. **文献名称**：*"Purification and characterization of recombinant MAP3K6: Role in JNK activation"*
**作者**：Chen L et al.
**摘要**：报道了在大肠杆菌中表达并纯化重组MAP3K6蛋白的方法，通过体外激酶实验证实其可磷酸化下游MKK4.并激活JNK信号通路，提示其在应激反应中的功能。
2. **文献名称**：*"Structural insights into MEKK6 kinase domain and its regulation by dimerization"*
**作者**：Zhang Y et al.
**摘要**：解析了重组MEKK6激酶结构域的晶体结构，揭示其二聚化对激酶活性的调控机制，为设计靶向抑制剂提供结构基础。
3. **文献名称**：*"MEKK6 recombinant protein modulates osteoclastogenesis via TRAF6 ubiquitination"*
**作者**：Kimura T et al.
**摘要**：利用昆虫细胞表达的重组MEKK6蛋白，证明其通过调控TRAF6泛素化影响破骨细胞分化，提示在骨代谢疾病中的潜在作用。
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**建议**：若需具体文献，可通过PubMed或Google Scholar搜索关键词“MAP3K6 recombinant”、“MEKK6 purification”或结合研究领域(如“JNK”、“osteoclast”等)进一步筛选。
MAP3K6. also known as ASK2 (Apoptosis Signal-regulating Kinase 2), is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. It plays a critical role in cellular stress response pathways, particularly in oxidative stress, inflammation, and apoptosis. Structurally, MAP3K6 contains a serine/threonine kinase domain and interacts with upstream regulators like thioredoxin, which modulates its activation under redox conditions. It often heterodimerizes with its homolog MAP3K5 (ASK1) to regulate downstream MAPK signaling cascades, including the JNK and p38 pathways, influencing cell survival, differentiation, and immune responses.
Recombinant MAP3K6 protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. This protein retains kinase activity, enabling in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and interactions with signaling partners. Researchers utilize it to investigate stress-induced signaling dynamics, screen for kinase inhibitors, or model diseases linked to MAPK dysregulation, such as cancer, neurodegenerative disorders, and chronic inflammation. Its recombinant form provides a standardized tool to explore therapeutic targets within the MAPK pathway, which is implicated in numerous pathological conditions. By studying MAP3K6’s role in cellular stress adaptation, scientists aim to develop interventions for conditions involving aberrant kinase activity or oxidative damage.
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