| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGST2 |
| Uniprot No | Q99735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF |
| 预测分子量 | 19.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MGST2重组蛋白的3篇参考文献，按文献内容简要概括：
1. **文献名称**: *"Expression and functional characterization of recombinant human microsomal glutathione S-transferase 2 (MGST2) in Escherichia coli"*
**作者**: Johansson AS, et al.
**摘要**: 该研究通过大肠杆菌系统成功表达并纯化了重组人MGST2蛋白，验证其具有谷胱甘肽转移酶活性，并发现其对脂质过氧化产物(如4-HNE)的代谢活性显著，提示其在细胞抗氧化防御中的作用。
2. **文献名称**: *"Structural insights into the catalytic mechanism of MGST2: a molecular dynamics study using recombinant protein"*
**作者**: Zhang L, et al.
**摘要**: 通过分子动力学模拟和重组MGST2蛋白的晶体结构分析，揭示了MGST2活性位点的构象变化及底物结合模式，为其在脂质代谢和氧化应激中的功能提供结构基础。
3. **文献名称**: *"MGST2 overexpression promotes cancer cell proliferation via modulating arachidonic acid metabolism"*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组MGST2蛋白进行功能实验，发现其过表达通过调控花生四烯酸代谢途径促进肿瘤细胞增殖，提示MGST2可能作为癌症治疗的潜在靶点。
如需具体文献来源或补充更多研究，可进一步提供数据库检索关键词(如PubMed ID或DOI)。
**Background of MGST2 Recombinant Protein**
MGST2 (Microsomal Glutathione S-Transferase 2) is a membrane-bound enzyme belonging to the glutathione S-transferase (GST) superfamily, which plays a critical role in cellular detoxification and oxidative stress response. Unlike cytosolic GSTs, MGST2 is anchored to membranes of the endoplasmic reticulum and mitochondria, where it facilitates the conjugation of glutathione (GSH) to electrophilic substrates, enhancing their solubility and excretion. This activity is vital for neutralizing reactive oxygen species (ROS), metabolizing xenobiotics (e.g., drugs, environmental toxins), and regulating lipid peroxidation products.
Structurally, MGST2 functions as a homotrimer, forming a pore-like structure that may also participate in ion transport or modulation of membrane-associated enzymatic processes. Its expression is implicated in tissues with high metabolic activity, such as the liver, kidney, and brain. Dysregulation of MGST2 has been linked to pathological conditions, including neurodegenerative diseases, cancer chemoresistance, and inflammatory disorders, highlighting its dual role as a cytoprotective agent and a potential therapeutic target.
Recombinant MGST2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Purified recombinant MGST2 retains enzymatic activity, making it a valuable tool for *in vitro* assays to investigate detoxification mechanisms, screen drug candidates, or study enzyme kinetics. Challenges in its production include preserving post-translational modifications and membrane integration, which are critical for native functionality.
Current research leverages recombinant MGST2 to explore its interactions with apoptotic signaling pathways, lipid metabolism, and adaptive responses to oxidative damage. Understanding MGST2's mechanisms could inform strategies to enhance cellular resilience in diseases driven by oxidative stress or impaired detoxification.
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