| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGST1 |
| Uniprot No | P10620 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-155aa |
| 氨基酸序列 | MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVFANPEDC VAFGKGENAKKYLRTDDRVERVRRAHLNDLENIIPFLGIGLLYSLSGPDP STAILHFRLFVGARIYHTIAYLTPLPQPNRALSFFVGYGVTLSMAYRLLK SKLYL |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MGST1重组蛋白的3篇参考文献示例(内容基于公开研究概括，非真实文献)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant Human Microsomal Glutathione S-Transferase 1 (MGST1) in *E. coli*"
**作者**: Andersson C, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功重组表达人源MGST1蛋白，并验证其酶活性。结果显示重组MGST1具有谷胱甘肽转移酶和过氧化物酶活性，对脂质过氧化产物具有显著代谢能力，为后续功能研究提供工具。
2. **文献名称**: "Structural Insights into MGST1: Cryo-EM Analysis of a Membrane-Associated Detoxification Enzyme"
**作者**: Holm PJ, et al.
**摘要**: 通过冷冻电镜技术解析了重组MGST1的三维结构，揭示其作为膜结合蛋白的寡聚化特征及活性位点构象，阐明了其在细胞膜上催化谷胱甘肽依赖性解毒反应的分子机制。
3. **文献名称**: "Role of MGST1 in Chemoresistance: Overexpression in Cancer Cells and Interaction with Anticancer Drugs"
**作者**: Singh S, et al.
**摘要**: 研究利用重组MGST1蛋白验证其在肿瘤细胞中的过表达现象，证明其通过代谢化疗药物(如烷化剂)降低药物毒性，揭示了MGST1介导的耐药性通路，为逆转耐药性提供靶点。
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注：以上文献为示例，实际引用时请以真实发表的论文为准(可通过PubMed/Google Scholar检索关键词如"MGST1 recombinant"或"Microsomal GST1 expression")。
**Background of MGST1 Recombinant Protein**
Microsomal glutathione S-transferase 1 (MGST1) is a membrane-associated enzyme belonging to the glutathione S-transferase (GST) superfamily, which plays a critical role in cellular detoxification and oxidative stress response. Unlike cytosolic GSTs, MGST1 is anchored to membranes of the endoplasmic reticulum and mitochondrial-associated membranes, contributing to its unique functional and structural properties. It catalyzes the conjugation of glutathione (GSH) to electrophilic substrates, facilitating the elimination of xenobiotics, lipid peroxidation byproducts, and reactive oxygen species (ROS).
MGST1 forms a homotrimeric structure, with each subunit (~17 kDa) containing a distinct active site that enables GSH-dependent enzymatic activity. This trimeric configuration enhances stability and allows interaction with hydrophobic substrates within lipid bilayers. Recombinant MGST1 protein is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, followed by purification via affinity chromatography. Its recombinant form retains native enzymatic activity, making it a valuable tool for *in vitro* studies.
Research highlights MGST1's involvement in critical pathways, including leukotriene and prostaglandin biosynthesis, which link it to inflammatory responses. It also modulates apoptosis by influencing mitochondrial membrane permeability. Dysregulation of MGST1 has been implicated in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and drug resistance, where its overexpression may protect malignant cells from chemotherapeutic agents.
The recombinant protein is widely used to study enzyme kinetics, substrate specificity, and inhibitor screening for therapeutic development. Additionally, it serves as a model to explore membrane protein biochemistry and oxidative stress mechanisms. Understanding MGST1's role in health and disease continues to drive interest in its structure-function relationships and potential as a drug target.
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