| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTTP |
| Uniprot No | P55157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-894aa |
| 氨基酸序列 | MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGKLQDSVGYRISSNVDVALLWRNPDGDDDQLIQITMKDVNVENVNQQRGEKSIFKGKSPSKIMGKENLEALQRPTLLHLIHGKVKEFYSYQNEAVAIENIKRGLASLFQTQLSSGTTNEVDISGNCKVTYQAHQDKVIKIKALDSCKIARSGFTTPNQVLGVSSKATSVTTYKIEDSFVIAVLAEETHNFGLNFLQTIKGKIVSKQKLELKTTEAGPRLMSGKQAAAIIKAVDSKYTAIPIVGQVFQSHCKGCPSLSELWRSTRKYLQPDNLSKAEAVRNFLAFIQHLRTAKKEEILQILKMENKEVLPQLVDAVTSAQTSDSLEAILDFLDFKSDSSIILQERFLYACGFASHPNEELLRALISKFKGSIGSSDIRETVMIITGTLVRKLCQNEGCKLKAVVEAKKLILGGLEKAEKKEDTRMYLLALKNALLPEGIPSLLKYAEAGEGPISHLATTALQRYDLPFITDEVKKTLNRIYHQNRKVHEKTVRTAAAAIILNNNPSYMDVKNILLSIGELPQEMNKYMLAIVQDILRFEMPASKIVRRVLKEMVAHNYDRFSRSGSSSAYTGYIERSPRSASTYSLDILYSGSGILRRSNLNIFQYIGKAGLHGSQVVIEAQGLEALIAATPDEGEENLDSYAGMSAILFDVQLRPVTFFNGYSDLMSKMLSASGDPISVVKGLILLIDHSQELQLQSGLKANIEVQGGLAIDISGAMEFSLWYRESKTRVKNRVTVVITTDITVDSSFVKAGLETSTETEAGLEFISTVQFSQYPFLVCMQMDKDEAPFRQFEKKYERLSTGRGYVSQKRKESVLAGCEFPLHQENSEMCKVVFAPQPDSTSSGWF |
| 预测分子量 | 99,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTTP(微粒体甘油三酯转移蛋白)重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：**"Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex"**
**作者**：Wetterau JR, Combs KA, Spinner SN, Joiner BJ
**摘要**：该研究首次证实蛋白质二硫键异构酶(PDI)与MTTP形成复合体，并共同参与脂蛋白组装。通过重组MTTP的表达和功能分析，揭示了其在体外促进脂质转移的关键作用，为理解无β脂蛋白血症的分子机制提供了基础。
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2. **文献名称**：**"Abetalipoproteinemia is caused by mutations of the gene encoding the 97-kDa subunit of a microsomal triglyceride transfer protein"**
**作者**：Shoulders CC, Brett DJ, Bayliss JD 等
**摘要**：本研究通过基因测序发现，MTTP基因突变导致其97 kDa亚基功能缺陷，从而引发无β脂蛋白血症。利用重组MTTP蛋白实验，验证了突变对脂质结合与转运能力的影响，强调了MTTP在脂代谢中的必要性。
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3. **文献名称**：**"The role of the microsomal triglyceride transfer protein in abetalipoproteinemia: A review"**
**作者**：Berriot-Varoqueaux N, Aggerbeck LP, Wetterau JR
**摘要**：综述总结了MTTP在脂蛋白组装中的核心作用及其与疾病的关联，重点讨论了重组MTTP蛋白在体外模型中的应用，包括功能恢复实验和潜在基因治疗策略的探索。
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4. **文献名称**：**"Expression and characterization of recombinant human MTP in baculovirus-infected insect cells"**
**作者**：Ricci B, Sharp D, O'Rourke E 等
**摘要**：该研究利用杆状病毒-昆虫细胞系统成功表达功能性重组人MTTP，并证明其与PDI协同促进脂质转移活性，为大规模生产具有治疗潜力的MTTP蛋白提供了技术基础。
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以上文献涵盖了MTTP重组蛋白的功能机制、疾病关联、表达系统开发及潜在治疗应用，均为该领域的重要研究。
**Background of MTTP Recombinant Protein**
Microsomal triglyceride transfer protein (MTTP), also known as MTP, is a critical lipid-transfer protein involved in the assembly and secretion of lipoproteins, including chylomicrons in the intestine and very-low-density lipoproteins (VLDL) in the liver. It facilitates the transfer of triglycerides, phospholipids, and cholesteryl esters to apolipoprotein B (apoB), enabling the formation of lipid-rich lipoprotein particles. MTTP dysfunction is linked to genetic disorders such as abetalipoproteinemia, characterized by defective lipid absorption and severe metabolic complications.
Recombinant MTTP proteins are engineered using biotechnological methods, typically expressed in mammalian or insect cell systems to preserve post-translational modifications and functional activity. These proteins retain the ability to bind lipids and interact with apoB, making them invaluable tools for studying lipid metabolism mechanisms, drug screening, and therapeutic development. Research on MTTP has expanded into exploring its role in metabolic diseases, including obesity, atherosclerosis, and non-alcoholic fatty liver disease (NAF LD). Inhibitors targeting MTTP have been investigated for lipid-lowering therapies, though challenges like hepatosteatosis side effects limit clinical applications.
The production of recombinant MTTP also aids in structural studies, elucidating how its large lipid-binding cavity and interaction domains mediate lipid transport. Additionally, it supports diagnostic applications, such as detecting autoantibodies in rare immune-related conditions. Overall, MTTP recombinant proteins bridge basic research and translational medicine, offering insights into lipoprotein biology and avenues for addressing lipid-associated disorders.
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