| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAST2 |
| Uniprot No | Q6P0Q8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAST2重组蛋白的3篇模拟参考文献(基于领域知识推测，供参考)：
1. **文献名称**：*"Structural and functional analysis of MAST2 kinase domain reveals its regulation by N-terminal motifs"*
**作者**：Chen Y. et al.
**摘要**：研究解析了MAST2激酶结构域的重组蛋白晶体结构，发现其N端调控基序通过自抑制机制调控激酶活性，为靶向MAST2的药物设计提供了结构基础。
2. **文献名称**：*"MAST2-PTEN interaction modulates cell migration via PI3K/AKT pathway"*
**作者**：Garcia-Rodriguez J.L. et al.
**摘要**：通过重组MAST2蛋白与PTEN的体外结合实验，证实MAST2直接磷酸化PTEN，抑制其脂质磷酸酶活性，从而增强PI3K/AKT信号通路并促进肿瘤细胞迁移。
3. **文献名称**：*"Recombinant MAST2 kinase production in E. coli and its application in tau phosphorylation studies"*
**作者**：Wang H. et al.
**摘要**：报道了利用大肠杆菌系统高效表达可溶性MAST2重组激酶的方法，并证明其在体外可磷酸化阿尔茨海默病相关tau蛋白的特定位点，提示MAST2在神经退行性疾病中的潜在作用。
**备注**：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台以"MAST2 recombinant protein"或"MAST2 kinase purification"为关键词检索最新论文。
MAST2 (Microtubule-Associated Serine/Threonine-Protein Kinase 2) is a member of the MAST kinase family, characterized by a unique N-terminal domain, a serine/threonine kinase domain, and a C-terminal PDZ domain. It plays a role in cellular signaling, cytoskeletal regulation, and intracellular trafficking by interacting with various partners, including scaffolding proteins, receptors, and cytoskeletal components. MAST2 is implicated in modulating pathways such as NF-κB, PTEN, and JNK, influencing processes like inflammation, apoptosis, and cell proliferation. Dysregulation of MAST2 has been linked to neurological disorders, cancer, and immune-related conditions, making it a potential therapeutic target.
Recombinant MAST2 proteins are engineered in vitro using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional MAST2 for research. These proteins retain key domains, enabling studies on kinase activity, protein-protein interactions, and signaling mechanisms. Researchers use them to investigate MAST2's role in disease models, screen inhibitors, or validate substrates. For example, recombinant MAST2 helps elucidate its interaction with PTEN in tumor suppression or its phosphorylation of tau in neurodegenerative contexts. Its production often includes tags (e.g., GST, His) for purification and detection. As MAST2 gains attention in drug discovery, recombinant variants serve as critical tools for structural analysis and targeted therapy development.
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