| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTN4 |
| Uniprot No | Q9NQC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1192aa |
| 氨基酸序列 | MEDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEEEEDEDEDLEELEVLERKPAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPARPPPPPPASVSPQAEPVWTPPAPAPAAPPSTPAAPKRRGSSGSVDETLFALPAASEPVIRSSAENMDLKEQPGNTISAGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNLSTVLPTEGTLQENVSEASKEVSEKAKTLLIDRDLTEFSELEYSEMGSSFSVSPKAESAVIVANPREEIIVKNKDEEEKLVSNNILHNQQELPTALTKLVKEDEVVSSEKAKDSFNEKRVAVEAPMREEYADFKPFERVWEVKDSKEDSDMLAAGGKIESNLESKVDKKCFADSLEQTNHEKDSESSNDDTSFPSTPEGIKDRSGAYITCAPFNPAATESIATNIFPLLGDPTSENKTDEKKIEEKKAQIVTEKNTSTKTSNPFLVAAQDSETDYVTTDNLTKVTEEVVANMPEGLTPDLVQEACESELNEVTGTKIAYETKMDLVQTSEVMQESLYPAAQLCPSFEESEATPSPVLPDIVMEAPLNSAVPSAGASVIQPSSSPLEASSVNYESIKHEPENPPPYEEAMSVSLKKVSGIKEEIKEPENINAALQETEAPYISIACDLIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSDDSIPDVPQKQDETVMLVKESLTETSFESMIEYENKEKLSALPPEGGKPYLESFKLSLDNTKDTLLPDEVSTLSKKEKIPLQMEELSTAVYSNDDLFISKEAQIRETETFSDSSPIEIIDEFPTLISSKTDSFSKLAREYTDLEVSHKSEIANAPDGAGSLPCTELPHDLSLKNIQPKVEEKISFSDDFSKNGSATSKVLLLPPDVSALATQAEIESIVKPKVLVKEAEKKLPSDTEKEDRSPSAIFSAELSKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE |
| 预测分子量 | 129,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RTN4重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**: *"Expression and purification of recombinant RTN4/Nogo protein in Escherichia coli"*
**作者**: Chen et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达和纯化重组RTN4蛋白的优化方法，通过His标签亲和层析获得高纯度蛋白，并验证其抑制神经突生长的生物学活性。
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2. **标题**: *"Structural analysis of the RTN4 C-terminal domain reveals its role in endoplasmic reticulum membrane shaping"*
**作者**: Park et al.
**摘要**: 利用重组RTN4的C端结构域进行X射线晶体学分析，揭示了其通过疏水结构域与内质网膜相互作用，调控膜曲率和细胞器形态的分子机制。
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3. **标题**: *"Recombinant RTN4B as a therapeutic target in autoimmune neuroinflammation"*
**作者**: Smith et al.
**摘要**: 研究重组RTN4B蛋白在多发性硬化小鼠模型中的作用，发现其通过抑制T细胞活化减轻炎症反应，提示其作为自身免疫性神经疾病治疗靶点的潜力。
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如需更多文献，可进一步限定研究领域(如神经再生、癌症等)。
**Background of RTN4 Recombinant Protein**
RTN4 (Reticulon 4), also known as Nogo, is a member of the reticulon protein family characterized by its conserved reticulon homology domain (RHD) with two hydrophobic regions forming hairpin structures. It is primarily expressed in the endoplasmic reticulum (ER) and plays roles in membrane curvature, vesicle trafficking, and ER shaping. RTN4 gained prominence due to its neuronal isoform, Nogo-A, which inhibits axon regeneration in the central nervous system (CNS) by interacting with the Nogo-66 receptor (NgR1) and paired immunoglobulin-like receptor B (PIRB). This inhibitory function contributes to the limited regenerative capacity of CNS neurons after injury.
Three major isoforms (Nogo-A, -B, -C) arise from alternative splicing. Nogo-A is predominantly expressed in the CNS, while Nogo-B and -C are found in various tissues, including vascular and immune cells. Beyond neural repair, RTN4 isoforms regulate apoptosis, cell adhesion, and angiogenesis. Nogo-B, for example, promotes vascular repair via interaction with the sphingosine-1-phosphate receptor (S1PR2).
Recombinant RTN4 proteins are engineered to study these diverse functions. Produced using bacterial, insect, or mammalian expression systems, they retain specific functional domains (e.g., Nogo-66 loop for receptor binding). These proteins are crucial for elucidating RTN4’s molecular mechanisms, screening therapeutic agents (e.g., neutralizing antibodies for spinal cord injury), and exploring roles in diseases like cancer and autoimmune disorders. Challenges include maintaining post-translational modifications and isoform-specific activity during production. Current research focuses on modulating RTN4 signaling to enhance neural repair and target pathological processes.
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