| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLU |
| Uniprot No | P10909 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-449aa |
| 氨基酸序列 | DQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIREGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE |
| 预测分子量 | 52.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CLU(Clusterin)重组蛋白相关的文献示例及摘要概括：
1. **文献名称**：*Recombinant Clusterin Protein Attenuates Neuronal Apoptosis via PI3K/Akt Signaling Pathway*
**作者**：Zhang et al.
**摘要**：研究利用重组CLU蛋白处理神经元细胞，发现其通过激活PI3K/Akt通路抑制氧化应激诱导的细胞凋亡，提示CLU在神经退行性疾病中的潜在治疗作用。
2. **文献名称**：*Structural and Functional Characterization of Recombinant Human Clusterin*
**作者**：Klockenbusch & Kast
**摘要**：通过重组技术表达并纯化人源CLU蛋白，解析其三维结构，证实其分子伴侣功能及与β-淀粉样蛋白的相互作用，为阿尔茨海默病机制研究提供依据。
3. **文献名称**：*Role of Recombinant Clusterin in Chemoresistance of Ovarian Cancer Cells*
**作者**：Nuutinen et al.
**摘要**：发现重组CLU通过抑制Bax/Bak依赖的线粒体凋亡通路增强卵巢癌细胞对顺铂的耐药性，提示靶向CLU可能改善化疗效果。
4. **文献名称**：*Recombinant Secretory Clusterin (sCLU) Promotes Cancer Cell Metastasis via EMT Modulation*
**作者**：Yerbury et al.
**摘要**：证明重组分泌型CLU通过调控上皮-间质转化(EMT)相关蛋白(如E-cadherin和Snail)促进乳腺癌细胞侵袭转移，揭示其在肿瘤进展中的关键作用。
以上文献方向涵盖神经保护、结构解析、化疗耐药及肿瘤转移，可反映CLU重组蛋白的多功能研究。实际文献需通过PubMed或Web of Science以关键词“recombinant clusterin”检索。
**Background of CLU (Clusterin) Recombinant Protein**
CLU, commonly known as Clusterin or apolipoprotein J, is a multifunctional glycoprotein encoded by the *CLU* gene. Initially identified in the 1980s, CLU gained attention due to its ubiquitous expression in tissues and involvement in diverse physiological and pathological processes. Structurally, mature CLU is a secreted heterodimeric glycoprotein formed by two subunits (α and β) linked by disulfide bonds. It exhibits chaperone-like activity, aiding in protein folding, aggregation prevention, and cellular stress response.
CLU plays dual roles in apoptosis, acting as either a pro-survival or pro-death factor depending on cellular context. It is implicated in lipid transport, immune regulation, and tissue remodeling. Pathologically, CLU is associated with cancer progression, neurodegenerative diseases (e.g., Alzheimer’s), atherosclerosis, and age-related disorders. Its overexpression in tumors often correlates with therapy resistance, while reduced levels in neurodegenerative conditions suggest a protective role.
Recombinant CLU proteins are produced using expression systems (e.g., mammalian, bacterial, or yeast) to study its molecular mechanisms or therapeutic potential. These proteins retain functional properties, enabling research into CLU's interaction with pathogens, amyloid proteins, or cell-surface receptors. Purification typically involves affinity chromatography, ensuring high purity and bioactivity.
Current applications include exploring CLU as a disease biomarker, a target for cancer therapy, or a tool to modulate protein aggregation in neurodegeneration. Its recombinant form also aids in drug discovery, screening compounds that enhance or inhibit its activity. Despite its complexity, CLU remains a promising focus for understanding disease mechanisms and developing targeted interventions.
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