| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXTL1 |
| Uniprot No | Q92935 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676aa |
| 氨基酸序列 | MQSWRRRKSLWLALSASWLLLVLLGGFSLLRLALPPRPRPGASQGWPRWLDAELLQSFSQPGELPEDAVSPPQAPHGGSCNWESCFDTSKCRGDGLKVFVYPAVGTISETHRRILASIEGSRFYTFSPAGACLLLLLSLDAQTGECSSMPLQWNRGRNHLVLRLHPAPCPRTFQLGQAMVAEASPTVDSFRPGFDVALPFLPEAHPLRGGAPGQLRQHSPQPGVALLALEEERGGWRTADTGSSACPWDGRCEQDPGPGQTQRQETLPNATFCLISGHRPEAASRFLQALQAGCIPVLLSPRWELPFSEVIDWTKAAIVADERLPLQVLAALQEMSPARVLALRQQTQFLWDAYFSSVEKVIHTTLEVIQDRIFGTSAHPSLLWNSPPGALLALSTFSTSPQDFPFYYLQQGSRPEGRFSALIWVGPPGQPPLKLIQAVAGSQHCAQILVLWSNERPLPSRWPETAVPLTVIDGHRKVSDRFYPYSTIRTDAILSLDARSSLSTSEVDFAFLVWQSFPERMVGFLTSSHFWDEAHGGWGYTAERTNEFSMVLTTAAFYHRYYHTLFTHSLPKALRTLADEAPTCVDVLMNFIVAAVTKLPPIKVPYGKQRQEAAPLAPGGPGPRPKPPAPAPDCINQIAAAFGHMPLLSSRLRLDPVLFKDPVSVQRKKYRSLEKP |
| 预测分子量 | 74,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXTL1重组蛋白的简要参考文献示例(内容基于领域常识模拟整理，非真实文献)：
1. **文献名称**：*Recombinant EXTL1 Protein Expression and Its Role in Heparan Sulfate Biosynthesis*
**作者**：Kimura T, et al.
**摘要**：研究报道了人源EXTL1重组蛋白在大肠杆菌中的高效表达与纯化，证实其具有α1.4-N-乙酰氨基葡萄糖转移酶活性，参与硫酸乙酰肝素链的延伸调控。
2. **文献名称**：*Structural Characterization of Recombinant EXTL1 and Its Interaction with EXT2*
**作者**：Saito M, et al.
**摘要**：通过X射线晶体学解析EXTL1重组蛋白的结构，发现其与EXT2形成复合物，提出EXTL1在糖基转移酶复合体组装中的辅助功能机制。
3. **文献名称**：*Functional Analysis of EXTL1 Knockout Cells Using Recombinant Protein Rescue*
**作者**：Chen L, et al.
**摘要**：利用重组EXTL1蛋白回补实验，证明其在细胞增殖和Hedgehog信号通路中的关键作用，缺失导致硫酸乙酰肝素合成缺陷及信号传导异常。
4. **文献名称**：*Enzymatic Activity Screening of EXTL1 Recombinant Variants in Cancer Models*
**作者**：Gupta R, et al.
**摘要**：构建多种EXTL1重组突变体，发现其酶活性的改变与肿瘤细胞侵袭性相关，提示EXTL1可能通过调节细胞表面多糖影响癌症进展。
注：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed/Web of Science)检索确认。
EXTL1 (Exostosin-like 1) is a member of the EXT family of glycosyltransferases, which play critical roles in heparan sulfate (HS) biosynthesis. HS, a linear polysaccharide ubiquitously expressed on cell surfaces and in the extracellular matrix, is essential for regulating cellular signaling, adhesion, and interactions. The EXT family includes EXTL1-3 and EXT1/2. with EXTL1 sharing structural homology but distinct functional roles. It is primarily involved in the initiation and elongation of HS chains by catalyzing the transfer of N-acetylglucosamine (GlcNAc) residues, a key step in forming the HS backbone.
Recombinant EXTL1 protein is engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its enzymatic activity, structure, and interactions. Its production enables detailed biochemical analyses, including substrate specificity studies and inhibitor screening. Structurally, EXTL1 contains a conserved glycosyltransferase domain critical for its catalytic function. Unlike EXT1/2. which form a heterocomplex for HS polymerization, EXTL1 may function independently or synergistically with other enzymes in early HS synthesis stages.
Research on EXTL1 has implications for understanding developmental disorders and cancers. Mutations in EXT family genes are linked to hereditary multiple exostoses (HME), a skeletal disorder characterized by benign bone tumors. While EXTL1 mutations are rare in HME, its dysregulation has been observed in certain cancers, suggesting roles in tumor suppression or progression via HS-dependent signaling pathways (e.g., Wnt, FGF). Recombinant EXTL1 also serves as a tool to investigate HS-mediated pathogen entry, angiogenesis, and tissue homeostasis, offering potential therapeutic targets for HS-related pathologies. Its study bridges glycobiology and disease mechanisms, emphasizing the importance of HS in health and disease.
×
