| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOP3 |
| Uniprot No | Q13472 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1001aa |
| 氨基酸序列 | MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR |
| 预测分子量 | 112,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOP3重组蛋白(假设为拓扑异构酶III)的3篇参考文献概览：
1. **文献名称**：*Role of Topoisomerase IIIα in DNA Replication and Recombination*
**作者**：Wallis, J.W., et al.
**摘要**：探讨了TOP3α与BLM解旋酶协同作用，在DNA复制过程中解决复制叉停滞及异常重组结构的机制，强调了其在维持基因组稳定性中的关键角色。
2. **文献名称**：*Meiotic Function of TOP3β in Crossover Formation*
**作者**：Li, X., & Wang, H.
**摘要**：研究TOP3β在小鼠减数分裂中的功能，发现其通过调控双链断裂修复途径影响交叉重组频率，缺失导致配子形成异常及不育。
3. **文献名称**：*Expression and Purification of Recombinant Human TOP3 in E. coli*
**作者**：Zhang, Y., et al.
**摘要**：开发了高效大肠杆菌表达系统，优化TOP3重组蛋白的可溶性和纯化流程，并验证其体外切割-连接DNA的酶活性，为功能研究提供基础。
4. **文献名称**：*TOP3 Mutations and Cancer Susceptibility*
**作者**：Johnson, R.D., & Patel, D.
**摘要**：通过全基因组关联分析，发现TOP3基因家族突变与乳腺癌和卵巢癌风险增加相关，提示其在肿瘤发生中的潜在抑癌功能。
注：上述文献为示例性质，具体内容需以实际发表研究为准。若需真实文献，建议通过PubMed或Google Scholar检索关键词“Topoisomerase III recombination”或“TOP3 recombinant protein”。
TOP3 (Topoisomerase III) is a member of the type IA topoisomerase family, essential for resolving DNA topological stress during replication, repair, and recombination. It catalyzes the passage of single-stranded DNA through transient breaks in complementary strands, relieving negative supercoils and decatenating intertwined DNA molecules. In eukaryotes, two isoforms, TOP3α and TOP3β, exist. TOP3α is highly conserved from yeast to humans and interacts with helicases like BLM (mutated in Bloom syndrome) to resolve recombination intermediates, particularly in replication fork restart and meiosis. TOP3β, while structurally similar, has distinct roles, including potential involvement in RNA metabolism and neurological functions.
Recombinant TOP3 proteins are produced via heterologous expression systems (e.g., E. coli, insect cells) for functional studies. Their purified forms enable biochemical assays to dissect enzymatic mechanisms, substrate preferences, and interactions with partner proteins. For instance, recombinant human TOP3α-BLM complexes have been instrumental in modeling Holliday junction dissolution. Structural studies using recombinant TOP3 proteins, often combined with cryo-EM or X-ray crystallography, reveal conserved catalytic cores with Rossmann-fold domains and unique C-terminal extensions that mediate partner binding.
Therapeutic interest in TOP3 stems from its role in genome stability; dysregulation links to cancer and neurodegenerative disorders. Recombinant TOP3 variants with specific mutations (e.g., catalytic tyrosines) serve as tools to study disease mechanisms or screen topoisomerase-targeting drugs. Recent CRISPR-based studies also utilize recombinant TOP3 for precise genome editing applications. Despite functional overlaps with other topoisomerases, TOP3's unique partnership with helicases positions it as a critical player in managing replication-associated genomic stress.
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