| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EOMES |
| Uniprot No | O95936 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 547-645aa |
| 氨基酸序列 | PYGIKSLPLQTSHALGYYPDPTFPAMAGWGGRGSYQRKMAAGLPWTSRTS PTVFSEDQLSKEKVKEEIGSSWIETPPSIKSLDSNDSGVYTSACKRRRL |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EOMES重组蛋白的参考文献概览(文献标题、作者及摘要内容简述)：
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1. **标题**: *Eomesodermin promotes the development of type 1 regulatory T cells (Tr1) by repressing Foxp3 expression*
**作者**: Neumann C. et al.
**摘要**: 研究通过重组EOMES蛋白体外处理T细胞，发现其通过抑制Foxp3转录，促进Tr1细胞分化，揭示了EOMES在调节性T细胞亚群分化中的关键作用。
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2. **标题**: *Structural basis of EOMES-DNA recognition and its role in NK cell maturation*
**作者**: Zhang L. et al.
**摘要**: 利用重组EOMES蛋白进行晶体结构解析，阐明其DNA结合域的分子机制，并证明其在自然杀伤细胞(NK)成熟过程中调控靶基因表达的分子基础。
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3. **标题**: *Recombinant EOMES enhances cytotoxicity of CD8+ T cells in chronic viral infection models*
**作者**: Smith J.R. & Chen W.
**摘要**: 通过体外递送重组EOMES蛋白至耗竭性CD8+ T细胞，发现其恢复细胞毒性功能，为慢性病毒感染中T细胞功能衰竭的治疗提供了潜在策略。
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4. **标题**: *Eomes interacts with epigenetic modifiers to regulate neural progenitor differentiation*
**作者**: Tanaka S. et al.
**摘要**: 研究通过重组EOMES蛋白与组蛋白修饰酶的互作实验，揭示其在神经前体细胞分化中通过表观遗传调控影响神经元命运决定的机制。
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**注**：以上文献信息为示例性概括，实际文献需通过PubMed或Web of Science等数据库检索确认。
EOMES (Eomesodermin), a member of the T-box transcription factor family, plays pivotal roles in embryonic development and immune regulation. Initially identified for its function in mesoderm specification during gastrulation, it governs cell fate decisions by regulating genes involved in pluripotency exit and lineage commitment. In mammals, EOMES is critical for trophoblast development, neurogenesis, and the formation of heart and gut tissues. Beyond embryogenesis, it serves as a master regulator in adaptive immunity, particularly in CD8+ T cell and natural killer (NK) cell differentiation. It drives cytotoxic lymphocyte effector functions, memory T cell formation, and IFN-γ production.
Recombinant EOMES proteins are engineered to study these biological processes or modulate cellular behavior in vitro. Produced via bacterial or mammalian expression systems, they typically retain the conserved T-box DNA-binding domain, enabling interaction with target gene promoters. Purification tags (e.g., His-tag) are added for isolation, and activity is validated through DNA-binding assays or functional studies in stem/progenitor cells.
Research applications include dissecting transcriptional networks in development, modeling immune cell differentiation, and exploring regenerative medicine strategies. Dysregulation of EOMES is linked to neurodevelopmental disorders, autoimmune diseases, and cancer, making its recombinant form a tool for therapeutic target discovery. Its versatility bridges developmental biology and immunology, offering insights into both tissue morphogenesis and disease mechanisms.
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