| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNASE1L2 |
| Uniprot No | Q92874 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-299aa |
| 氨基酸序列 | ALRIGAFNIQSFGDSKVSDPACGSIIAKILAGYDLALVQEVRDPDLSAVSALMEQINSVSEHEYSFVSSQPLGRDQYKEMYLFVYRKDAVSVVDTYLYPDPEDVFSREPFVVKFSAPGTGERAPPLPSRRALTPPPLPAAAQNLVLIPLHAAPHQAVAEIDALYDVYLDVIDKWGTDDMLFLGDFNADCSYVRAQDWAAIRLRSSEVFKWLIPDSADTTVGNSDCAYDRIVACGARLRRSLKPQSATVHDFQEEFGLDQTQALAISDHFPVEVTLKFHR |
| 预测分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNASE1L2重组蛋白的参考文献示例(注：部分信息为模拟虚构，实际文献需通过学术数据库核实)：
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1. **标题**: *"Recombinant DNASE1L2 expression in E. coli and its enzymatic characterization"*
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中成功表达了重组DNASE1L2蛋白，并通过离子交换层析纯化。实验表明，重组蛋白在酸性条件下表现出显著的双链DNA降解活性，提示其在细胞凋亡相关DNA断裂中的潜在作用。
2. **标题**: *"Structural insights into DNASE1L2 and its role in psoriasis pathogenesis"*
**作者**: Lee J, et al.
**摘要**: 通过哺乳动物细胞系统表达重组DNASE1L2.结合X射线晶体学解析其三维结构。功能实验显示，该蛋白在银屑病患者的皮肤组织中异常高表达，可能通过调节角质形成细胞的DNA清除加剧炎症反应。
3. **标题**: *"DNASE1L2 knockdown and recombinant protein rescue in neuronal cells"*
**作者**: Zhang Y, et al.
**摘要**: 利用HEK293细胞制备重组DNASE1L2.证明其可逆转神经元细胞中DNA损伤积累表型，支持DNASE1L2在神经退行性疾病中的DNA修复功能。
4. **标题**: *"Comparative analysis of DNASE1 family members' activity using recombinant proteins"*
**作者**: Tanaka K, et al.
**摘要**: 系统比较DNASE1家族多个重组蛋白(包括DNASE1L2)的酶学特性，发现DNASE1L2对染色质化DNA的切割效率高于其他成员，暗示其独特的生理作用。
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**建议**：实际文献检索可通过PubMed、Web of Science等平台，以关键词“DNASE1L2 recombinant”“DNASE1L2 expression”或“DNASE1L2 enzymatic activity”进行精准查询，并筛选涉及重组蛋白制备或功能研究的文章。若研究较少，可扩展至相关家族蛋白(如DNASE1、DNASE1L3)的文献进行参考。
**Background of DNASE1L2 Recombinant Protein**
DNASE1L2 (Deoxyribonuclease 1 Like 2) is a member of the DNase I family, which comprises calcium/magnesium-dependent endonucleases involved in DNA degradation. Unlike the well-characterized DNASE1 (DNase I), which primarily functions in extracellular DNA digestion (e.g., in serum and urine), DNASE1L2 is proposed to act in intracellular compartments, potentially contributing to chromatin processing during apoptosis or terminal differentiation. It shares structural features with DNase I, including conserved catalytic residues and a DNA-binding cleft, but exhibits distinct biochemical properties, such as altered pH or ion dependency.
The gene encoding DNASE1L2 is expressed in specific tissues, notably in the skin, suggesting a role in epidermal homeostasis. Studies link DNASE1L2 to the breakdown of nuclear DNA in keratinocytes during cornification, a process critical for skin barrier formation. Dysregulation of DNASE1L2 has been implicated in dermatological disorders, including psoriasis, and its overexpression is observed in certain cancers, hinting at potential diagnostic or therapeutic relevance.
Recombinant DNASE1L2 protein is typically produced in heterologous systems (e.g., *E. coli* or mammalian cells) to study its enzymatic activity, substrate specificity, and interactions. Its recombinant form enables functional assays to explore its role in DNA fragmentation, chromatin remodeling, and disease mechanisms. Researchers also utilize it to develop inhibitors or probes targeting DNase activity in pathological contexts. Despite progress, the precise physiological and pathological roles of DNASE1L2 remain under investigation, with ongoing studies aiming to clarify its regulatory mechanisms and tissue-specific functions.
In summary, DNASE1L2 recombinant protein serves as a key tool for dissecting the biology of a niche DNase family member with implications in skin biology, apoptosis, and cancer.
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