| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTNR1B |
| Uniprot No | P49286 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MSENGSFANCCEAGGWAVRPGWSGAGSARPSRTPRPPWVAPALSAVLIVTTAVDVVGNLLVILSVLRNRKLRNAGNLFLVSLALADLVVAFYPYPLILVAIFYDGWALGEEHCKASAFVMGLSVIGSVFNITAIAINRYCYICHSMAYHRIYRRWHTPLHICLIWLLTVVALLPNFFVGSLEYDPRIYSCTFIQTASTQYTAAVVVIHFLLPIAVVSFCYLRIWVLVLQARRKAKPESRLCLKPSDLRSFLTMFVVFVIFAICWAPLNCIGLAVAINPQEMAPQIPEGLFVTSYLLAYFNSCLNAIVYGLLNQNFRREYKRILLALWNPRHCIQDASKGSHAEGLQSPAPPIIGVQHQADAL |
| 预测分子量 | 40,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTNR1B重组蛋白的3篇参考文献示例(注：文献信息为虚构，仅作格式参考)：
1. **文献名称**: "Functional Expression and Characterization of Human MTNR1B in Baculovirus-Insect Cell System"
**作者**: Zhang L, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达了人源MTNR1B重组蛋白，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组MTNR1B能够特异性结合褪黑素，并激活下游cAMP信号通路，为研究该受体的药理学特性提供了工具。
2. **文献名称**: "Structural Insights into MTNR1B Receptor Activation by Melatonin Analogs"
**作者**: Thompson R, et al.
**摘要**: 通过冷冻电镜解析MTNR1B重组蛋白与褪黑素类似物的复合物结构，揭示了受体配体结合的关键氨基酸残基及构象变化机制。研究为开发靶向MTNR1B的糖尿病治疗药物提供了结构基础。
3. **文献名称**: "Optimization of MTNR1B Recombinant Protein Expression in Mammalian Cells for Drug Screening"
**作者**: Chen H, et al.
**摘要**: 在HEK293细胞中优化MTNR1B的稳定表达体系，开发了基于荧光素酶报告基因的高通量药物筛选平台。研究发现，特定单核苷酸多态性(如rs10830963)显著影响受体对激动剂的响应活性。
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**说明**：实际文献需通过PubMed/Google Scholar等平台检索关键词(如“MTNR1B recombinant protein”或“melatonin receptor expression”)。真实研究多关注该受体在糖尿病、昼夜节律中的功能及其基因变异的影响。
**Background of MTNR1B Recombinant Protein**
MTNR1B (Melatonin Receptor 1B) is a G protein-coupled receptor (GPCR) encoded by the *MTNR1B* gene, primarily expressed in the retina, pancreas, and brain. It binds melatonin, a hormone regulating circadian rhythms, sleep-wake cycles, and seasonal physiological adaptations. MTNR1B, alongside MTNR1A, mediates melatonin's effects by coupling with Gαi/o proteins, inhibiting cAMP production, and modulating downstream signaling pathways.
Genetic studies link *MTNR1B* variants to metabolic disorders, particularly type 2 diabetes (T2D). Single nucleotide polymorphisms (SNPs) near or within *MTNR1B* (e.g., rs10830963) are associated with elevated fasting glucose levels and impaired insulin secretion, suggesting a role in pancreatic β-cell function. This connection has driven research into MTNR1B's molecular mechanisms in glucose homeostasis and its potential as a therapeutic target.
Recombinant MTNR1B protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor protein for *in vitro* studies. Its applications include ligand-binding assays, structural analysis (e.g., X-ray crystallography or cryo-EM), and screening melatoninergic compounds for drug development. Recombinant MTNR1B also aids in studying receptor dimerization, post-translational modifications, and signaling crosstalk with other metabolic pathways.
Despite progress, challenges remain in understanding tissue-specific signaling and reconciling genetic associations with functional impacts. Further research using recombinant MTNR1B could clarify its role in circadian-metabolic interplay and inform precision therapies for diabetes or sleep disorders.
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