| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYP |
| Uniprot No | P08247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MLLLADMDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYS GELQLNVDCANKTESDLSIEVEFEYPFRLHQVYFDAPTCRGGTTKVFLVG DYSSSAEFFVTVAVFAFLYSMGALATYIFLQNKYRENNKGPMLDFLATAV FAFMWLVSSSAWAKGLSDVKMATDPENIIKEMPVCRQTGNTCKELRDLVT SGLNTSVVFGFLNLVLWVGNLWFVFKETGWAAPFLRAPPGAPEKQPAPGD AYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPAGSGGSGYGPQGDYGQQ GYGPQGAPTSFSNQM |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYP(Synaptophysin)重组蛋白的3篇参考文献示例，涵盖表达、结构及功能研究方向：
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1. **文献名称**：*"Expression and Purification of Recombinant Synaptophysin in Escherichia coli for Synaptic Vesicle Studies"*
**作者**：Zhang L, et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达重组SYP蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其与突触小泡膜的结合活性，为体外研究突触传递机制提供工具。
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2. **文献名称**：*"Structural Insights into Synaptophysin-Caveolin Interaction Using Recombinant Protein Co-expression"*
**作者**：Müller R, et al.
**摘要**：通过共表达SYP与Caveolin-1重组蛋白，解析了二者在细胞膜微结构域中的相互作用界面，揭示了SYP在膜融合和信号转导中的潜在分子机制。
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3. **文献名称**：*"Functional Characterization of Recombinant Synaptophysin in a Neuronal Cell Model of Parkinson’s Disease"*
**作者**：Chen H, et al.
**摘要**：利用HEK293细胞表达重组SYP，证明其在帕金森病模型中能够部分恢复多巴胺能神经元的突触囊泡循环功能，提示SYP可能作为神经退行性疾病的治疗靶点。
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注：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索最新具体文献。
SYP (Synaptophysin) is a well-characterized integral membrane protein predominantly found in the synaptic vesicles of neurons, playing a critical role in neurotransmission and synaptic plasticity. As a member of the synaptophysin/physin family, it consists of four transmembrane domains and forms a hexameric complex, interacting with other synaptic proteins like synaptobrevin to regulate vesicle fusion, neurotransmitter release, and recycling. Its expression is largely restricted to neuroendocrine cells, making it a widely used biomarker for identifying neuronal and neuroendocrine tumors in clinical diagnostics.
Recombinant SYP proteins are engineered using genetic cloning techniques, where the SYP gene is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems) to produce purified proteins for research and therapeutic applications. This recombinant approach allows for high-yield, scalable production while maintaining structural and functional integrity. Researchers utilize recombinant SYP to study synaptic mechanisms, model neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and screen drugs targeting synaptic dysfunction. Its role in vesicle dynamics also makes it relevant in studying psychiatric disorders linked to synaptic impairment.
Beyond neuroscience, SYP recombinant proteins are employed in antibody development for immunohistochemistry and ELISA assays, enhancing diagnostic precision. Recent advances in cryo-EM and structural biology have further clarified SYP’s molecular interactions, informing therapies aimed at modulating synaptic activity. Despite its established utility, challenges remain in mimicking post-translational modifications in heterologous expression systems, driving ongoing optimization of recombinant platforms. Overall, SYP recombinant proteins serve as indispensable tools bridging basic neurobiology and translational medicine.
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