| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMMR |
| Uniprot No | O75330 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-724aa |
| 氨基酸序列 | MSFPKAPLKR FNDPSGCAPS PGAYDVKTLE VLKGPVSFQK SQRFKQQKES KQNLNVDKDT TLPASARKVK SSESKESQKN DKDLKILEKE IRVLLQERGA QDRRIQDLET ELEKMEARLN AALREKTSLS ANNATLEKQL IELTRTNELL KSKFSENGNQ KNLRILSLEL MKLRNKRETK MRGMMAKQEG MEMKLQVTQR SLEESQGKIA QLEGKLVSIE KEKIDEKSET EKLLEYIEEI SCASDQVEKY KLDIAQLEEN LKEKNDEILS LKQSLEENIV ILSKQVEDLN VKCQLLEKEK EDHVNRNREH NENLNAEMQN LKQKFILEQQ EREKLQQKEL QIDSLLQQEK ELSSSLHQKL CSFQEEMVKE KNLFEEELKQ TLDELDKLQQ KEEQAERLVK QLEEEAKSRA EELKLLEEKL KGKEAELEKS SAAHTQATLL LQEKYDSMVQ SLEDVTAQFE SYKALTASEI EDLKLENSSL QEKAAKAGKN AEDVQHQILA TESSNQEYVR MLLDLQTKSA LKETEIKEIT VSFLQKITDL QNQLKQQEED FRKQLEDEEG RKAEKENTTA ELTEEINKWR LLYEELYNKT KPFQLQLDAF EVEKQALLNE HGAAQEQLNK IRDSYAKLLG HQNLKQKIKH VVKLKDENSQ LKSEVSKLRC QLAKKKQSET KLQEELNKVL GIKHFDPSKA FHHESKENFA LKTPLKEGNT NCYRAPMECQ ESWK |
| 预测分子量 | 84,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMMR(RHAMM)重组蛋白的3篇代表性文献摘要概述：
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1. **文献名称**: *RHAMM regulates cell migration via interaction with the dynein light chain DYNLL1*
**作者**: Maxwell CA et al. (2005)
**摘要**: 研究通过重组表达RHAMM蛋白片段，揭示了其与动力蛋白轻链DYNLL1的相互作用机制，证明该互作在调控微管稳定性及细胞迁移中的关键作用，为癌症转移机制提供新见解。
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2. **文献名称**: *Recombinant RHAMM induces mitotic defects and genomic instability in breast cancer cells*
**作者**: Tolg C et al. (2010)
**摘要**: 利用重组RHAMM蛋白处理乳腺癌细胞，发现其通过干扰中心体功能导致染色体分离异常和基因组不稳定性，提示RHAMM过表达可能促进肿瘤恶性进展。
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3. **文献名称**: *HMMR as a novel biomarker for prognosis in hepatocellular carcinoma*
**作者**: Li Y et al. (2018)
**摘要**: 通过重组HMMR蛋白建立体外模型，验证其作为肝细胞癌预后标志物的潜力，发现HMMR高表达与患者生存率降低显著相关，机制涉及EMT通路激活。
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**备注**：若需具体文献链接或补充信息，可进一步说明研究方向(如结构解析/药物靶点等)。
**Background of HMMR Recombinant Protein**
HMMR (Hyaluronan-Mediated Motility Receptor), also known as RHAMM (Receptor for Hyaluronan-Mediated Motility), is a multifunctional protein initially identified for its role in cell motility and hyaluronan (HA) signaling. It binds to hyaluronan, a major component of the extracellular matrix, and regulates cellular processes such as migration, proliferation, and adhesion. Structurally, HMMR lacks a transmembrane domain but interacts with cell surface receptors like CD44 and growth factor receptors (e.g., EGFR), modulating downstream signaling pathways, including ERK and FAK.
In normal physiology, HMMR is critical during embryogenesis and wound healing. However, its dysregulation is strongly linked to cancer progression. Overexpression of HMMR correlates with tumor aggressiveness, metastasis, and therapy resistance in cancers like breast, colorectal, and glioblastoma. It promotes genomic instability by interacting with mitotic spindle components and facilitates invasive behavior via HA-mediated pathways.
Recombinant HMMR protein, produced in systems like *E. coli* or mammalian cells, enables in vitro studies to dissect its molecular mechanisms. Researchers use it to investigate HA-binding domains, receptor interactions, and signaling crosstalk. Truncated variants or tagged forms (e.g., His-tagged, GFP-fusion) aid in structural analyses, antibody development, or inhibitor screening. In therapeutic contexts, recombinant HMMR serves as a tool to validate targeting strategies, such as blocking HA-HMMR interactions to suppress metastasis.
Despite its potential, challenges remain in understanding isoform-specific functions and post-translational modifications. Ongoing research aims to clarify HMMR's dual roles in homeostasis and disease, positioning it as a promising biomarker and therapeutic target. The availability of recombinant HMMR accelerates these efforts, bridging mechanistic insights to clinical applications.
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