| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIPK1 |
| Uniprot No | Q86Z02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1210aa |
| 氨基酸序列 | MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQANSSHQVANFNIPAYDQGLLLPAPAVEHIVVTAADSSGSAATSTFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVDSNGSVQIIEEHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSTWQQLPGVALHNSVQPTAMIPEAMGSGQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLDVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPSSSGLKPRSNVISYVTVNDSPDSDSSLSSPYSTDTLSALRGNSGSVLEGPGRVVADGTGTRTIIVPPLKTQLGDCTVATQASGLLSNKTKPVASVSGQSSGCCITPTGYRAQRGGTSAAQPLNLSQNQQSSAAPTSQERSSNPAPRRQQAFVAPLSQAPYTFQHGSPLHSTGHPHLAPAPAHLPSQAHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL |
| 预测分子量 | 130 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIPK1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant HIPK1 Kinase Domain: Expression, Purification, and Substrate Specificity Analysis"*
**作者**：Li X, Wang Y, et al.
**摘要**：研究报道了人源HIPK1激酶结构域的重组表达及纯化方法，通过大肠杆菌系统获得高纯度蛋白，并验证其对p53蛋白的体外磷酸化活性，揭示了HIPK1在DNA损伤信号中的潜在调控机制。
2. **文献名称**：*"Structural Insights into HIPK1 Activation by Oligomerization"*
**作者**：Zhang R, Chen L, et al.
**摘要**：利用冷冻电镜解析了重组HIPK1蛋白的寡聚化结构，发现其自激活依赖于N端结构域的相互作用，为HIPK1激酶活性调控提供了结构基础。
3. **文献名称**：*"HIPK1 Recombinant Protein Enhances Apoptosis in Oxidative Stress Models"*
**作者**：Kim S, Park JH, et al.
**摘要**：通过哺乳动物细胞表达系统获得功能性HIPK1重组蛋白，实验证明其过表达可显著增强H₂O₂诱导的细胞凋亡，提示HIPK1在氧化应激反应中的促凋亡作用。
(注：上述文献为模拟概括，实际引用时需以具体数据库检索结果为准。)
**Background of HIPK1 Recombinant Protein**
HIPK1 (Homeodomain-Interacting Protein Kinase 1) is a serine/threonine kinase belonging to the HIPK family, which includes HIPK1. HIPK2. HIPK3. and HIPK4. It plays a regulatory role in diverse cellular processes, including apoptosis, cell proliferation, DNA damage response, and transcriptional regulation. HIPK1 interacts with transcription factors, chromatin modifiers, and signaling molecules, influencing pathways such as Hippo, Wnt, and TGF-β. Its activity is modulated by post-translational modifications, including phosphorylation and ubiquitination, which regulate subcellular localization and stability.
Recombinant HIPK1 protein is engineered for in vitro studies to dissect its biochemical properties, substrate specificity, and interaction networks. It is typically produced using expression systems like *E. coli*, insect cells, or mammalian cells, followed by purification via affinity chromatography. The recombinant form retains kinase activity, enabling researchers to investigate its role in signaling cascades, disease mechanisms (e.g., cancer, neurodegeneration, diabetic complications), and therapeutic targeting.
HIPK1 dysregulation has been linked to pathologies; for example, its overexpression is observed in certain cancers, while reduced activity correlates with neurodegenerative conditions. Studies using recombinant HIPK1 have advanced drug discovery efforts, particularly in designing kinase inhibitors or activators. Additionally, structural analyses of the recombinant protein help map functional domains, such as the kinase domain and the homeodomain-binding region, providing insights into substrate recognition and regulatory mechanisms.
Overall, HIPK1 recombinant protein serves as a critical tool for unraveling its biological functions and exploring its potential as a diagnostic or therapeutic target.
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