| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST1 |
| Uniprot No | P0DMM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-295aa |
| 氨基酸序列 | MELIQDTSRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLINTYPKSGTTWVSQILDMIYQGGDLEKCNRAPIYVRVPFLEVNDPGEPSGLETLKDTPPPRLIKSHLPLALLPQTLLDQKVKVVYVARNPKDVAVSYYHFHRMEKAHPEPGTWDSFLEKFMAGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGRSLPEETMDFMVQHTSFKEMKKNPMTNYTTVPQELMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL |
| 预测分子量 | 50.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于ST1重组蛋白相关研究的模拟参考文献示例(请注意，这些为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：Expression and Purification of Recombinant ST1 Enterotoxin in E. coli
**作者**：Zhang L., et al.
**摘要**：本研究报道了利用大肠杆菌表达系统高效表达ST1重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证了其热稳定性及生物学活性。
2. **文献名称**：Structural Analysis of ST1 Toxin and Its Receptor Binding Mechanism
**作者**：Wang Y., Smith J.R.
**摘要**：通过X射线晶体学解析ST1蛋白的三维结构，揭示了其与肠道细胞表面受体结合的特定结构域，为抗毒素药物设计提供了理论基础。
3. **文献名称**：Development of a ST1-Based Vaccine Candidate Against Enterotoxigenic Bacteria
**作者**：Chen H., et al.
**摘要**：评估了重组ST1蛋白作为疫苗抗原的免疫原性，动物实验显示其能诱导中和抗体并有效减少细菌感染引起的腹泻症状。
4. **文献名称**：A Rapid Detection Kit for ST1 Toxin Using Recombinant Antibodies
**作者**：Kim S., et al.
**摘要**：基于ST1重组蛋白制备单克隆抗体，开发出快速检测试剂盒，可在临床样本中灵敏检测ST1毒素，适用于食品安全及疾病诊断。
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**提示**：实际研究中建议通过PubMed、Web of Science等平台，以关键词“ST1 recombinant protein”、“ST1 enterotoxin”或具体蛋白全称(如heat-stable enterotoxin ST1)检索最新文献。若涉及特定物种或疾病，需进一步限定关键词。
**Background of ST1 Recombinant Protein**
ST1 recombinant protein is a genetically engineered protein derived from the strategic modification and expression of the ST1 gene, which encodes a biologically active molecule involved in specific cellular processes. Originally identified in studies focusing on [insert relevant field, e.g., immune regulation, pathogen-host interactions, or signaling pathways], the ST1 protein has garnered attention for its functional roles, such as [mention key functions: e.g., modulating immune responses, facilitating cell adhesion, or acting as a receptor ligand]. Recombinant technology allows large-scale production of ST1 in heterologous systems like *E. coli*, yeast, or mammalian cell lines, ensuring high purity and consistency for research or therapeutic applications.
The design of ST1 recombinant protein often incorporates tags (e.g., His-tag, GST) to simplify purification and detection. Structural analyses reveal domains critical for its activity, such as [describe domains: e.g., a ligand-binding domain or enzymatic motif]. Its recombinant form retains native conformation and functionality, validated through assays like ELISA, western blot, or functional cell-based tests.
ST1’s applications span multiple fields. In biomedical research, it serves as a tool to study [specific pathways or diseases]. Therapeutically, it is explored for [potential uses: e.g., vaccine development, targeted drug delivery, or as a biologic drug]. Industrial uses include diagnostic kits or biosensors leveraging its binding specificity.
Challenges in ST1 production include optimizing expression yields, minimizing aggregation, and ensuring post-translational modifications in eukaryotic systems. Ongoing research focuses on enhancing stability and bioactivity through protein engineering. As a versatile biologic, ST1 recombinant protein exemplifies the convergence of genetic engineering and translational science, holding promise for addressing unmet needs in healthcare and biotechnology.
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