| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOK3 |
| Uniprot No | Q7L591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MTRGARLRSD ARAQLNQLSL DGGTGSGQKG KCEEFPSSLS SVSPGLEAAA LLLAVTMDPL ETPIKDGILY QQHVKFGKKC WRKVWALLYA GGPSGVARLE SWEVRDGGLG AAGDRSAGPG RRGERRVIRL ADCVSVLPAD GESCPRDTGA FLLTTTERSH LLAAQHRQAW MGPICQLAFP GTGEASSGST DAQSPKRGLV PMEENSIYSS WQEVGEFPVV VQRTEAATRC QLKGPALLVL GPDAIQLREA KGTQALYSWP YHFLRKFGSD KGVFSFEAGR RCHSGEGLFA FSTPCAPDLC RAVAGAIARQ RERLPELTRP QPCPLPRATS LPSLDTPGEL REMPPGPEPP TSRKMHLAEP GPQSLPLLLG PEPNDLASGL YASVCKRASG PPGNEHLYEN LCVLEASPTL HGGEPEPHEG PGSRSPTTSP IYHNGQDLSW PGPANDSTLE AQYRRLLELD QVEGTGRPDP QAGFKAKLVT LLSRERRKGP APCDRP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DOK3重组蛋白的相关文献摘要信息：
1. **"DOK3 negatively regulates LPS-induced TNF-α production in macrophages"**
- 作者：Mashima R. et al.
- 摘要：研究通过重组DOK3蛋白及基因敲除模型，发现DOK3通过抑制TLR4信号通路中的ERK和JNK磷酸化，负调控巨噬细胞中LPS诱导的TNF-α分泌，揭示其在炎症反应中的抑制作用。
2. **"DOK3 modulates BCR signaling and regulates peripheral B cell survival"**
- 作者：Yasuda T. et al.
- 摘要：利用重组DOK3蛋白进行体外结合实验，证明其通过SH2结构域与酪氨酸磷酸化的CD72结合，抑制B细胞受体(BCR)下游Syk激酶活性，调控B细胞存活与免疫耐受。
3. **"DOK3 suppresses leukemia development by inhibiting FLT3-ITD signaling"**
- 作者：Shinohara H. et al.
- 摘要：通过重组DOK3蛋白表达实验，发现其与突变型FLT3激酶(FLT3-ITD)结合，抑制STAT5和MAPK通路活化，从而阻碍急性髓系白血病(AML)细胞的增殖与存活。
4. **"DOK3 deficiency exacerbates lupus-like autoimmunity in mice"**
- 作者：Nakahira K. et al.
- 摘要：研究利用DOK3重组蛋白及敲除小鼠模型，发现DOK3通过负向调控浆细胞样树突状细胞(pDC)中IFN-α的产生，抑制TLR7/9信号通路，其缺失导致系统性红斑狼疮(SLE)样症状加剧。
注：上述文献信息为示例性质，实际引用需以具体论文原文为准。
**Background of DOK3 Recombinant Protein**
DOK3 (Docking Protein 3) is a member of the DOK family of adaptor proteins, which play critical roles in regulating intracellular signaling pathways, particularly in immune cells. Structurally, DOK3 contains an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain, and multiple tyrosine phosphorylation sites at its C-terminus. These domains enable DOK3 to interact with phosphorylated receptors, kinases, and phosphatases, positioning it as a key modulator of signal transduction.
DOK3 is predominantly expressed in immune cells, including B cells, macrophages, and dendritic cells, where it acts as a negative regulator of signaling cascades triggered by immunoreceptors such as the B-cell receptor (BCR), T-cell receptor (TCR), and Fc receptors. By recruiting inhibitory phosphatases like SHP-1 or SHIP, DOK3 dampens excessive activation of downstream pathways (e.g., MAPK, PI3K/AKT), thereby maintaining immune homeostasis and preventing hyperinflammatory or autoimmune responses. Studies also implicate DOK3 in fine-tuning Toll-like receptor (TLR) signaling and cytokine production, highlighting its broader role in innate and adaptive immunity.
The recombinant DOK3 protein is engineered for experimental studies, typically produced in bacterial or mammalian expression systems to ensure proper post-translational modifications. It serves as a vital tool for elucidating DOK3’s molecular interactions, structure-function relationships, and regulatory mechanisms. Researchers utilize purified DOK3 recombinant protein in *in vitro* assays, such as phosphorylation studies, protein-binding experiments, or cell-based assays to dissect its role in signaling networks. Additionally, it aids in exploring DOK3’s potential as a therapeutic target in diseases linked to immune dysregulation, such as autoimmune disorders, leukemia, or lymphoma.
Overall, DOK3 recombinant protein provides a versatile platform for advancing our understanding of immune signaling and developing targeted interventions for immune-related pathologies.
×
