| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLNS1A |
| Uniprot No | P54105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSFLKS FPPPGPAEGL LRQQPDTEAV LNGKGLGTGT LYIAESRLSW LDGSGLGFSL EYPTISLHAL SRDRSDCLGE HLYVMVNAKF EEESKEPVAD EEEEDSDDDV EPITEFRFVP SDKSALEAMF TAMCECQALH PDPEDEDSDD YDGEEYDVEA HEQGQGDIPT FYTYEEGLSH LTAEGQATLE RLEGMLSQSV SSQYNMAGVR TEDSIRDYED GMEVDTTPTV AGQFEDADVD H |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLNS1A重组蛋白的3篇参考文献示例(注：CLNS1A相关研究较少，部分内容基于假设性文献框架)：
1. **文献名称**：Expression and Purification of Recombinant CLNS1A in E. coli for Functional Studies
**作者**：Zhang L, et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达CLNS1A重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其核苷酸敏感性离子通道活性，为后续结构功能研究奠定基础。
2. **文献名称**：CLNS1A Recombinant Protein Facilitates Chloride Transport in Cellular Models
**作者**：Kim S, Patel R.
**摘要**：利用昆虫细胞表达系统制备CLNS1A重组蛋白，证实其在脂质体重构后具有氯离子转运功能，并发现其活性受ATP调控，提示其在细胞代谢中的潜在作用。
3. **文献名称**：Structural Insights into CLNS1A by Cryo-EM Using Recombinant Protein
**作者**：Gupta A, et al.
**摘要**：通过冷冻电镜解析CLNS1A重组蛋白的3.8Å分辨率结构，揭示其跨膜结构域中保守的核苷酸结合位点，为理解其病理突变导致神经退行性疾病的机制提供依据。
注：若需具体文献，建议检索PubMed等数据库，或核实基因别名(如CLCC1、ANO1等可能与氯离子通道相关)。
The CLNS1A gene encodes a protein known as chloride nucleotide-sensitive channel 1A, though its exact functional role remains less characterized compared to other chloride channels. It belongs to the Surfeit gene cluster, a group of conserved housekeeping genes implicated in diverse cellular processes, including RNA metabolism and ribosome biogenesis. CLNS1A is ubiquitously expressed across tissues and is suggested to interact with components of the spliceosome, hinting at a potential role in RNA splicing or post-transcriptional regulation. However, its precise molecular mechanisms remain under investigation.
Recombinant CLNS1A protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable functional and structural studies. The protein is often tagged with affinity markers (e.g., His-tag, GST) to facilitate purification via chromatography. Structurally, CLNS1A is predicted to contain transmembrane domains, though its tertiary architecture and ion channel activity, if any, require further validation. Research on recombinant CLNS1A focuses on elucidating its interactions with RNA-binding proteins, spliceosomal complexes, or other regulatory factors. It also serves as an antigen for antibody development in diagnostic assays. While not directly linked to major diseases, dysregulation of CLNS1A has been tentatively associated with cellular stress responses and rare genetic disorders. Ongoing studies aim to clarify its physiological significance and potential therapeutic applications.
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