| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAT4 |
| Uniprot No | Q14765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 氨基酸序列 | MSQWNQVQQL EIKFLEQVDQ FYDDNFPMEI RHLLAQWIEN QDWEAASNNE TMATILLQNL LIQLDEQLGR VSKEKNLLLI HNLKRIRKVL QGKFHGNPMH VAVVISNCLR EERRILAAAN MPVQGPLEKS LQSSSVSERQ RNVEHKVAAI KNSVQMTEQD TKYLEDLQDE FDYRYKTIQT MDQSDKNSAM VNQEVLTLQE MLNSLDFKRK EALSKMTQII HETDLLMNTM LIEELQDWKR RQQIACIGGP LHNGLDQLQN CFTLLAESLF QLRRQLEKLE EQSTKMTYEG DPIPMQRTHM LERVTFLIYN LFKNSFVVER QPCMPTHPQR PLVLKTLIQF TVKLRLLIKL PELNYQVKVK ASIDKNVSTL SNRRFVLCGT NVKAMSIEES SNGSLSVEFR HLQPKEMKSS AGGKGNEGCH MVTEELHSIT FETQICLYGL TIDLETSSLP VVMISNVSQL PNAWASIIWY NVSTNDSQNL VFFNNPPPAT LSQLLEVMSW QFSSYVGRGL NSDQLHMLAE KLTVQSSYSD GHLTWAKFCK EHLPGKSFTF WTWLEAILDL IKKHILPLWI DGYVMGFVSK EKERLLLKDK MPGTFLLRFS ESHLGGITFT WVDHSESGEV RFHSVEPYNK GRLSALPFAD ILRDYKVIMA ENIPENPLKY LYPDIPKDKA FGKHYSSQPC EVSRPTERGD KGYVPSVFIP ISTIRSDSTE PHSPSDLLPM SPSVYAVLRE NLSPTTIETA MKSPYSAE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAT4重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "Cloning and Functional Characterization of Recombinant STAT4 for T-cell Signaling Studies"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了人源STAT4基因的克隆、重组蛋白表达及纯化方法，利用哺乳动物表达系统获得高活性STAT4蛋白，并验证其在体外促进Th1细胞分化的功能，为免疫信号通路研究提供工具。
2. **文献名称**: "STAT4 Recombinant Protein Ameliorates Autoimmunity in Murine Lupus Models"
**作者**: Kimura T, et al.
**摘要**: 通过构建STAT4重组蛋白，在系统性红斑狼疮(SLE)小鼠模型中验证其治疗潜力，发现其能抑制过度活化的干扰素-γ通路，减轻肾脏病理损伤，提示STAT4靶向治疗的潜在价值。
3. **文献名称**: "Structural Insights into STAT4 Phosphorylation and Dimerization via Recombinant Protein Crystallography"
**作者**: Patel RK, et al.
**摘要**: 利用重组STAT4蛋白进行晶体结构解析，揭示其酪氨酸磷酸化位点及二聚化机制，阐明IL-12信号传导中构象变化的分子基础，为设计STAT4抑制剂提供结构依据。
4. **文献名称**: "Recombinant STAT4 Fusion Protein Enhances Antiviral Response in Human Macrophages"
**作者**: Costa M, et al.
**摘要**: 开发STAT4与穿透性肽融合的重组蛋白，证明其可有效激活巨噬细胞中干扰素调节因子(IRF)通路，增强抗病毒基因表达，为感染性疾病治疗提供新策略。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“STAT4 recombinant protein”或结合具体研究领域筛选。
STAT4 (Signal Transducer and Activator of Transcription 4) is a critical transcription factor involved in mediating cellular responses to cytokines, particularly interleukin-12 (IL-12) and type I interferons. It plays a pivotal role in regulating immune functions, including T-cell differentiation, Th1 immune responses, and the production of interferon-gamma (IFN-γ). Structurally, STAT4 contains conserved SH2 and DNA-binding domains, enabling its activation via phosphorylation by JAK kinases upon cytokine receptor engagement. Once activated, STAT4 forms dimers, translocates to the nucleus, and drives the expression of target genes essential for immune cell proliferation, inflammation, and antiviral defense.
Recombinant STAT4 protein is engineered using biotechnological methods, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) and validated for functional activity, such as phosphorylation and DNA-binding capacity. This recombinant tool is widely used in research to dissect JAK-STAT signaling mechanisms, study immune dysregulation in autoimmune diseases (e.g., rheumatoid arthritis, lupus), and explore cancer immunotherapy strategies. Its role in promoting pro-inflammatory pathways has also made STAT4 a potential therapeutic target, with inhibitors under investigation for autoimmune conditions. Conversely, enhancing STAT4 activity is explored to bolster antitumor immunity. As a reagent, recombinant STAT4 enables *in vitro* studies of protein-protein interactions, signaling crosstalk, and high-throughput drug screening, advancing both basic science and translational applications.
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