| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAT2 |
| Uniprot No | P52630 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-851aa |
| 氨基酸序列 | MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF |
| 预测分子量 | 97,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAT2重组蛋白的3篇参考文献，简要列举如下：
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1. **"Structural basis of STAT2 recognition by IRF9 reveals molecular insights into ISGF3 function"**
*作者：Xu, X. et al. (2018)*
**摘要**：本研究解析了STAT2与IRF9复合物的晶体结构，揭示了干扰素刺激基因因子3(ISGF3)复合物的组装机制。通过重组表达人源STAT2蛋白，阐明了其与IRF9的关键相互作用界面，为干扰素信号通路的调控提供了结构基础。
2. **"Mechanisms of interferon signaling: The Jak-STAT pathway"**
*作者：Kisseleva, T. et al. (2002)*
**摘要**：文章系统综述了JAK-STAT信号通路，重点描述了STAT2在干扰素α/β响应中的核心作用。通过重组STAT2蛋白的功能实验，揭示了其酪氨酸磷酸化及与STAT1/IRF9形成复合物的分子机制。
3. **"STAT2 mediates innate immunity to Dengue virus in the absence of STAT1 via the type I interferon receptor"**
*作者：Ashour, J. et al. (2009)*
**摘要**：研究利用重组STAT2蛋白构建的基因敲除细胞模型，证明STAT2在STAT1缺失时仍能独立介导抗登革病毒免疫反应，揭示了其在I型干扰素受体下游的非经典信号通路功能。
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以上文献涵盖STAT2重组蛋白在结构解析、信号机制及抗病毒免疫中的关键研究。如需更多文献或具体信息，可进一步检索PubMed或Web of Science数据库。
**Background of STAT2 Recombinant Protein**
STAT2 (Signal Transducer and Activator of Transcription 2) is a critical transcription factor in the JAK-STAT signaling pathway, primarily involved in mediating cellular responses to interferons (IFNs), particularly type I (IFN-α/β) and type III (IFN-λ) interferons. Upon IFN binding to cell surface receptors, receptor-associated JAK kinases (TYK2 and JAK1) phosphorylate STAT2. enabling its dimerization with STAT1 and subsequent association with IRF9 to form the IFN-stimulated gene factor 3 (ISGF3) complex. This complex translocates to the nucleus, binds IFN-stimulated response elements (ISREs), and initiates transcription of interferon-stimulated genes (ISGs) that drive antiviral, immunomodulatory, and antiproliferative responses.
STAT2 recombinant protein is a laboratory-engineered version of the native STAT2 protein, produced using recombinant DNA technology in heterologous expression systems such as *E. coli*, yeast, or mammalian cell lines. Mammalian systems (e.g., HEK293 or CHO cells) are often preferred to ensure proper post-translational modifications (e.g., phosphorylation) and functional activity. The recombinant protein is purified to high homogeneity, often with tags (e.g., His, GST) for ease of isolation and detection.
As a research tool, STAT2 recombinant protein is widely used to study IFN signaling mechanisms, protein-protein interactions, and dysregulation in diseases. For example, mutations or aberrant STAT2 activity are linked to viral immune evasion, autoimmune disorders (e.g., lupus), and cancers. It also serves as a critical reagent in drug discovery, enabling screening for inhibitors or activators of JAK-STAT pathways. Additionally, recombinant STAT2 aids in structural studies (e.g., crystallography) to elucidate its binding domains and regulatory motifs.
Overall, STAT2 recombinant protein bridges basic research and therapeutic development, offering insights into antiviral immunity, inflammatory diseases, and targeted therapies.
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