| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFHX4 |
| Uniprot No | Q86UP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-93aa |
| 氨基酸序列 | ETCDSPPISRQENGQSTSKLCGTTQLDNEVPEKVAGMEPDRENSSTDDNL KTDERKSEALLGFSVENAAATQVTSAKEIPCNECATSFPSLQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZFHX4重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *ZFHX4 promotes glioblastoma tumorigenesis by transcriptional regulation of DNA repair pathways*
**作者**: Smith A, et al.
**摘要**: 本研究利用重组ZFHX4蛋白进行体外DNA结合实验，发现其通过调控ATM/ATR信号通路增强胶质瘤细胞对DNA损伤的修复能力，促进肿瘤耐药性。
2. **文献名称**: *Structural characterization of ZFHX4 and its interaction with NuRD complex*
**作者**: Li X, et al.
**摘要**: 通过重组ZFHX4蛋白的晶体结构解析，揭示其锌指结构域与NuRD复合物的相互作用机制，为表观遗传调控相关疾病提供潜在靶点。
3. **文献名称**: *ZFHX4 regulates neuronal differentiation via Sox2 suppression in vitro*
**作者**: Tanaka R, et al.
**摘要**: 研究利用重组ZFHX4蛋白处理神经干细胞，证明其通过抑制Sox2转录活性，促进神经元分化和突触形成，提示其在神经发育中的作用。
4. **文献名称**: *High-yield purification and functional analysis of recombinant ZFHX4 in prostate cancer models*
**作者**: Chen L, et al.
**摘要**: 开发了重组ZFHX4蛋白的高效原核表达系统，并验证其在前列腺癌细胞中通过激活EMT通路促进转移的分子机制。
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**备注**：以上为模拟生成的参考文献，实际研究需以PubMed、Web of Science等平台检索关键词(如“ZFHX4 recombinant protein”“ZFHX4 function”)获取。
ZFHX4 (Zinc Finger Homeobox Protein 4) is a transcription factor belonging to the homeobox gene family, which plays critical roles in regulating developmental processes and cellular differentiation. Structurally, it contains N-terminal zinc finger motifs that mediate DNA binding and protein interactions, as well as a C-terminal homeobox domain responsible for sequence-specific DNA recognition. These features enable ZFHX4 to modulate gene expression by recruiting co-regulators to influence chromatin remodeling or transcriptional activation/repression.
Studies suggest ZFHX4 is involved in neural development, stem cell maintenance, and tumorigenesis. It is highly expressed in the brain, particularly in neural progenitor cells, where it may regulate cell fate decisions. Dysregulation of ZFHX4 has been linked to cancers, including glioblastoma, breast cancer, and hepatocellular carcinoma, where it often acts as an oncogene by promoting proliferation, invasion, or therapy resistance. For example, in glioblastoma, ZFHX4 interacts with chromatin remodelers like CHD4 to drive tumorigenic pathways.
Recombinant ZFHX4 protein is engineered using expression systems (e.g., E. coli, mammalian cells) for functional studies. Its purified form enables in vitro analyses, such as DNA-binding assays, protein interaction mapping, and structural studies. Researchers also use it to generate antibodies or screen for small molecules targeting its activity in disease contexts. Despite progress, ZFHX4’s full regulatory network and tissue-specific roles remain under investigation, highlighting the importance of recombinant tools in elucidating its biological and pathological mechanisms.
Overall, ZFHX4 recombinant protein serves as a vital resource for exploring its dual roles in development and disease, offering potential avenues for therapeutic intervention.
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