| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFHX1B |
| Uniprot No | O60315 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1214aa |
| 氨基酸序列 | MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPEEMKEDYDTMGPEATIQTAINNGTVKNANCTSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQYSNSRSPSLERSSKPLAPNSNPPTKDSLLPRSPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFSMNPFSAKPLYTALPPQSAFPPATFMPPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM |
| 预测分子量 | 136,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ZFHX1B(SIP1)重组蛋白的参考文献摘要信息：
1. **文献名称**: *ZFHX1B/SIP1: A multifunctional regulator of embryonic development and disease*
**作者**: Vandewalle C, et al.
**摘要**: 研究利用重组ZFHX1B蛋白揭示其通过结合E-cadherin启动子抑制上皮细胞粘附，并证实其通过TGF-β信号通路促进肿瘤细胞侵袭转移的分子机制。
2. **文献名称**: *Structural and functional analysis of the ZFHX1B transcriptional repressor in neural crest development*
**作者**: Higashi Y, et al.
**摘要**: 通过体外表达重组ZFHX1B蛋白，发现其锌指结构域特异性识别DNA靶序列，调控神经嵴细胞迁移相关基因(如MITF、Smad7)，解释了ZFHX1B突变导致Hirschsprung病的分子基础。
3. **文献名称**: *SIP1 interacts with c-Myb to modulate angiogenesis and tumor progression*
**作者**: Goossens S, et al.
**摘要**: 利用重组蛋白结合实验证明ZFHX1B与转录因子c-Myb直接互作，抑制血管生成相关基因(VEGF、PDGFRβ)表达，提示其在肿瘤微环境调控中的双重作用。
注：上述文献为示例性内容，实际文献需通过PubMed或Web of Science等平台检索。建议使用关键词“ZFHX1B recombinant protein”或“SIP1 protein purification”获取最新实验研究。
**Background of ZFHX1B Recombinant Protein**
ZFHX1B (Zinc Finger Homeobox 1B), also known as SIP1 (Smad Interacting Protein 1) or ZEB2 (Zinc Finger E-Box Binding Homeobox 2), is a transcription factor belonging to the ZEB protein family. It plays critical roles in embryonic development, cell differentiation, and epithelial-mesenchymal transition (EMT). Structurally, ZFHX1B contains two clusters of zinc finger motifs and a homeodomain, enabling DNA binding and interaction with co-regulators. It primarily functions as a transcriptional repressor, regulating target genes by binding to E-box sequences in promoter regions.
ZFHX1B is essential for neural crest cell migration and differentiation during embryogenesis. Its dysfunction is linked to Mowat-Wilson syndrome (MWS), a rare genetic disorder characterized by intellectual disability, distinct facial features, and congenital anomalies, caused by heterozygous mutations or deletions in the *ZFHX1B* gene. Beyond development, ZFHX1B is implicated in cancer progression, where it promotes metastasis by driving EMT and suppressing epithelial markers like E-cadherin.
Recombinant ZFHX1B protein is engineered for research to study its molecular mechanisms, interactions (e.g., with SMAD proteins in TGF-β/BMP signaling), and regulatory networks. Produced via bacterial or mammalian expression systems, the purified protein retains functional domains for in vitro assays, such as DNA-binding studies, chromatin immunoprecipitation (ChIP), or inhibitor screening. Its applications extend to disease modeling and therapeutic exploration, particularly in targeting EMT-related pathways in cancer or elucidating neurodevelopmental defects in MWS. Research on ZFHX1B also contributes to understanding epigenetic regulation, as it recruits histone deacetylases (HDACs) to modulate chromatin structure and gene expression.
Overall, ZFHX1B recombinant protein serves as a vital tool for dissecting its dual roles in development and disease, offering insights into potential therapeutic strategies.
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