| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLIC2 |
| Uniprot No | O15247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSGLRPGTQVDPEIELFVKAGSDGESIGNC PFCQRLFMILWLKGVKFNVTTVDMTRKPEELKDLAPGTNPPFLVYNKELK TDFIKIEEFLEQTLAPPRYPHLSPKYKESFDVGCNLFAKFSAYIKNTQKE ANKNFEKSLLKEFKRLDDYLNTPLLDEIDPDSAEEPPVSRRLFLDGDQLT LADCSLLPKLNIIKVAAKKYRDFDIPAEFSGVWRYLHNAYAREEFTHTCP EDKEIENTYANVAKQKS |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLIC2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Recombinant CLIC2 Protein Forms a Functional Chloride Channel with Unique pH Sensitivity"*
**作者**: Smith A, et al.
**摘要**: 该研究通过在大肠杆菌中表达并纯化重组CLIC2蛋白，发现其在体外能够形成功能性氯离子通道，并表现出独特的pH依赖性激活特性。研究揭示了CLIC2在酸性环境中的潜在生理作用。
2. **文献名称**: *"Structural Insights into CLIC2 Function: Role of the N-terminal Domain in Redox Regulation"*
**作者**: Brown L, et al.
**摘要**: 作者通过X射线晶体学解析了重组CLIC2蛋白的三维结构，发现其N端结构域在氧化还原调控中起关键作用，并提出了CLIC2可能通过构象变化调节离子通道活性的机制。
3. **文献名称**: *"CLIC2 Recombinant Protein Modulates Calcium Homeostasis in Cardiomyocytes"*
**作者**: Zhang Y, et al.
**摘要**: 该研究利用重组CLIC2蛋白进行细胞实验，证明其通过与心肌细胞钙处理蛋白(如RyR2)相互作用，调控细胞内钙稳态，提示CLIC2在心脏疾病中的潜在病理学意义。
(注：上述文献信息为示例，实际文献需通过数据库如PubMed检索确认。)
CLIC2 (Chloride Intracellular Channel 2) is a member of the CLIC protein family, which exhibits dual soluble and membrane-associated forms implicated in chloride ion transport and cellular homeostasis. Structurally, CLIC2 contains a glutathione S-transferase (GST)-like fold with a redox-sensitive domain, allowing it to transition between cytosolic and membranous states. It shares homology with other CLIC proteins but displays distinct tissue expression patterns, predominantly in the heart, skeletal muscle, and brain.
Functionally, CLIC2 regulates intracellular chloride flux, pH balance, and cell volume. It interacts with ryanodine receptors (RyRs) on the sarcoplasmic reticulum, modulating calcium release in excitable cells. This interaction suggests roles in cardiac and neuronal signaling. Dysregulation of CLIC2 has been linked to X-linked intellectual disability and epilepsy, particularly through mutations (e.g., p.His100Pro) that impair RyR1 binding, leading to disrupted calcium homeostasis and altered neuronal excitability.
Recombinant CLIC2 proteins are generated via bacterial or mammalian expression systems for functional studies. These proteins enable biochemical characterization, structural analysis (e.g., crystallography), and investigation of ion channel gating mechanisms. They also serve as tools to explore CLIC2’s role in diseases and potential therapeutic targeting. Notably, recombinant CLIC2 has been used to study its redox-dependent membrane insertion and oligomerization, providing insights into its dynamic regulation. Current research focuses on elucidating its pathophysiological mechanisms and validating its utility as a biomarker or drug target for neurological disorders.
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