| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOMM70A |
| Uniprot No | O94826 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-608aa |
| 氨基酸序列 | MAASKPVEAAVVAAAVPSSGSGVGGGGTAGPGTGGLPRWQLALAVGAPLLLGAGAIYLWSRQQRRREARGRGDASGLKRNSERKTPEGRASPAPGSGHPEGPGAHLDMNSLDRAQAAKNKGNKYFKAGKYEQAIQCYTEAISLCPTEKNVDLSTFYQNRAAAFEQLQKWKEVAQDCTKAVELNPKYVKALFRRAKAHEKLDNKKECLEDVTAVCILEGFQNQQSMLLADKVLKLLGKEKAKEKYKNREPLMPSPQFIKSYFSSFTDDIISQPMLKGEKSDEDKDKEGEALEVKENSGYLKAKQYMEEENYDKIISECSKEIDAEGKYMAEALLLRATFYLLIGNANAAKPDLDKVISLKEANVKLRANALIKRGSMYMQQQQPLLSTQDFNMAADIDPQNADVYHHRGQLKILLDQVEEAVADFDECIRLRPESALAQAQKCFALYRQAYTGNNSSQIQAAMKGFEEVIKKFPRCAEGYALYAQALTDQQQFGKADEMYDKCIDLEPDNATTYVHKGLLQLQWKQDLDRGLELISKAIEIDNKCDFAYETMGTIEVQRGNMEKAIDMFNKAINLAKSEMEMAHLYSLCDAAHAQTEVAKKYGLKPPTL |
| 预测分子量 | 67,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOMM70A重组蛋白的3篇示例参考文献(文献内容为虚构示例，仅作格式参考)：
1. **文献名称**: *Structural Insights into the Role of TOMM70A in Mitochondrial Protein Import*
**作者**: Stevens, A.R.G. et al.
**摘要**: 本研究解析了重组人源TOMM70A蛋白的晶体结构，揭示了其与线粒体前体蛋白及分子伴侣Hsp90的结合界面，阐明了TOMM70A通过构象变化介导蛋白质转运的分子机制。
2. **文献名称**: *TOMM70A Reconstitution in vitro Demonstrates Its Chaperone Activity for Tail-Anchored Proteins*
**作者**: Nayak, D. & Koehler, C.M.
**摘要**: 通过重组表达TOMM70A蛋白，结合体外重构实验，证明其作为分子脚手架参与线粒体膜尾锚定蛋白(如Fis1)的靶向识别，并依赖ATP水解调控底物释放。
3. **文献名称**: *Dysregulation of TOMM70A in Metabolic Disorders: Implications from Knockdown Models*
**作者**: Yan, J. et al.
**摘要**: 利用重组TOMM70A蛋白进行功能补偿实验，发现其表达水平下降导致细胞线粒体呼吸链复合体组装缺陷，与胰岛素抵抗和Ⅱ型糖尿病病理进程相关。
(注：以上文献为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索。)
TOMM70A (Translocase of Outer Mitochondrial Membrane 70A) is a crucial component of the mitochondrial protein import machinery. It is part of the TOM (Translocase of Outer Mitochondrial Membrane) complex, which facilitates the recognition and transport of nuclear-encoded proteins into mitochondria. Specifically, TOMM70A acts as a receptor for cytosolic chaperone-bound precursor proteins, particularly those targeting the inner mitochondrial membrane and matrix. Its structure includes tetratricopeptide repeat (TPR) domains that mediate interactions with client proteins and co-chaperones like HSP90.
Recombinant TOMM70A protein is engineered for in vitro studies to dissect mitochondrial import mechanisms, protein-protein interactions, and structural features. Produced using expression systems like *E. coli* or mammalian cells, it enables researchers to study its role in energy metabolism, apoptosis, and stress responses. Dysregulation of TOMM70A is linked to metabolic disorders, neurodegenerative diseases, and cancer, making it a target for therapeutic exploration.
The recombinant form is purified via affinity chromatography, often tagged with fluorescent or epitope markers for detection. Studies using this tool have revealed its ability to cooperate with TIM44 in preprotein sorting and its involvement in maintaining mitochondrial membrane potential. Additionally, TOMM70A’s interaction with viral proteins during infection highlights its broader cellular roles beyond protein import. Its recombinant version continues to advance drug discovery and mechanistic studies of mitochondrial dysfunction.
×
